LOCUS       sequence-85277-e        3962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3962)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmoglein1-C-18
FEATURES             Location/Qualifiers
     source          1..3962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..730
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
      301 cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa
      361 ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga
      481 gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa
      541 ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg
      781 aagcgactgg agtttcttca gagtagttgc agtgctgttc atttttctgg tggtggtaga
      841 agttaacagt gagttccgaa tccaggtaag agattataac actaaaaatg gcaccatcaa
      901 atggcattca atccgaaggc agaaacgtga atggatcaag ttcgcagcag cctgtcgtga
      961 aggtgaagac aactcaaaga ggaacccaat cgccaaaatt cactcagatt gtgctgcaaa
     1021 ccagcaagtt acataccgca tctctggagt aggaattgat cagccaccat atgggatctt
     1081 tgtcattaat cagaaaactg gtgaaattaa tataacatcc atagttgatc gagaggtcac
     1141 tcctttcttc attatctact gccgagctct gaactcaatg ggccaagatt tagagaggcc
     1201 tctagagctc agagtcaggg ttttggatat aaatgacaac cctccagtgt tttcaatggc
     1261 tacatttgca ggacaaatag aagaaaattc taatgcaaat acactggtga tgatactcaa
     1321 tgctactgac gcagatgaac cgaacaattt gaactcaaaa atagccttca agattataag
     1381 acaagaacct tcagattcac caatgtttat tatcaacaga aatactggag aaattcgaac
     1441 gatgaataat tttctagaca gagagcaata cggccagtat gctcttgctg taagaggctc
     1501 tgaccgagat ggtggggcag atggcatgtc agcggaatgt gagtgcaaca ttaaaatcct
     1561 cgatgtcaat gataatatcc cttacatgga acagtcttca tataccatag aaattcaaga
     1621 aaatactcta aattcaaatt tgctcgaaat tagagtaatt gatttggatg aagagttctc
     1681 agctaactgg atggcagtaa ttttctttat ctctggaaat gaaggaaatt ggtttgagat
     1741 agaaatgaat gaaagaacaa atgtgggaat tttaaaggtt gttaagccct tagattatga
     1801 agctatgcag agtctgcaac tcagtattgg tgtcagaaat aaagctgaat ttcatcattc
     1861 aattatgtct caatataaac tgaaagcatc tgcaatttct gtgactgtgt taaatgtaat
     1921 tgaaggccca gtgtttcgtc caggttcaaa gacatatgtt gtaactggta atatgggatc
     1981 aaatgataaa gtgggagact ttgtagctac tgacctggac acaggtagac cttcaacgac
     2041 tgttaggtat gtaatgggaa ataatccagc tgacctgctt gctgttgatt caagaacagg
     2101 caaactcact ttgaaaaata aagttaccaa ggaacagtac aatatgctcg gaggaaaata
     2161 ccaaggaacg attctctcta tagatgataa tcttcaaaga acttgcactg gtacaattaa
     2221 tattaacatt caaagttttg gtaatgacga caggactaat acagagccga acactaaaat
     2281 tactaccaat actggcagac aagaaagtac ttcttccact aactatgata ccagcacaac
     2341 ttctactgac tctagccaag tatattcttc tgaacccgga aacggagcca aagatttgtt
     2401 atcagacaat gtacattttg gtcctgctgg cattggactc ctcatcatgg gattcttggt
     2461 cttaggattg gtcccatttt tgatgatctg ttgtgattgt ggaggtgctc ctcgtagtgc
     2521 agctggcttt gagcctgttc ccgaatgttc agatggagca attcattcat gggcagtaga
     2581 aggaccacag cctgaaccca gggatataac cactgtcata ccacaaatac cacctgataa
     2641 cgcaaatata attgaatgca ttgacaactc aggagtttat acaaatgagt atggtggcag
     2701 agaaatgcaa gacctgggag gaggagagag aatgacagga tttgaactaa cagagggagt
     2761 taaaacttca ggaatgcctg agatatgtca agaatactct ggaacattaa gaagaaattc
     2821 tatgagggaa tgtagagaag gaggtctgaa tatgaatttc atggaaagct acttctgtca
     2881 gaaagcatat gcttacgcag atgaagatga aggacgccca tctaatgact gtttgctcat
     2941 atatgacatc gaaggtgtag gttcccctgc tggctctgtg ggttgttgta gcttcattgg
     3001 agaagacctg gatgacagct tcttggatac cctgggacct aaatttaaga agttggcaga
     3061 catcagccta ggaaaagaat catatccaga ccttgatcct tcttggccac cacaaagcac
     3121 tgaaccagtt tgccttcctc aggaaacaga gcccgttgtt agtggacacc caccaatctc
     3181 cccacatttc ggcactacca cagtaatttc tgagagcacc tatccctcgg gacctggtgt
     3241 actgcatcct aagcctattc tcgatcctct gggctatggt aatgtcactg tgaccgagtc
     3301 ttacaccacc tctgacactc tgaagccctc tgtgcacgtt cacgataacc gaccagcatc
     3361 aaacgtggta gtgacagaga gagtggtcgg cccaatctct ggcgctgatt tgcatggaat
     3421 gttagagatg cctgacttgc gagatgggtc gaatgttata gtgacagaaa gggtaatagc
     3481 accaagctct agtctaccca cctctctgac tatccatcat cctagagagt cttcaaatgt
     3541 ggtagtgaca gaaagagtaa tccaaccaac ttccggcatg ataggtagtc tgagtatgca
     3601 ccccgagtta gccaatgccc acaatgtcat tgtgacagag agggttgttt ctggtgctgg
     3661 cgtaactgga attagtggca ccactgggat cagcggtggc ataggcagca gtggcctggt
     3721 tggcaccagc atgggtgctg ggagcggtgc cctgagtgga gctggcataa gtggtggtgg
     3781 cattggcctg agcagcttgg gagggacagc cagcattggc cacatgagga gttcctctga
     3841 ccatcacttt aaccaaacca ttgggtccgc ctcccctagc acagctcgaa gtcgaatcac
     3901 aaagtatagt accgtgcaat atagcaagta gggatccacc ggatctagat aactgatcat
     3961 aa
//
