LOCUS       sequence-85279-e        4175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4175)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmoglein2-C-18
FEATURES             Location/Qualifiers
     source          1..4175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..733
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgctagcg ctaccgttcg ccaccatggt gagcaagggc gaggagaata acatggccat
       61 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa
      181 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      241 cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt
      301 ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt
      361 taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac
      421 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga
      481 ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct
      541 gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt
      601 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga
      661 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      721 cgagctgtac aagtccggaa gtggagctgg gagcggggca agcggaagcg gaagtggctc
      781 cggtggggcg cggagcccgg gacgcgcgta cgccctgctg cttctcctga tctgctttaa
      841 cgttggaagt ggacttcact tacaggtctt aagcacaaga aatgaaaata agctgcttcc
      901 taaacatcct catttagtgc ggcaaaagcg cgcctggatc accgcccccg tggctcttcg
      961 ggagggagag gatctgtcca agaagaatcc aattgccaag atacattctg atcttgcaga
     1021 agaaagagga ctcaaaatta cttacaaata cactggaaaa gggattacag agccaccttt
     1081 tggtatattt gtctttaaca aagatactgg agaactgaat gttaccagca ttcttgatcg
     1141 agaagaaaca ccattttttc tgctaacagg ttacgctttg gatgcaagag gaaacaatgt
     1201 agagaaaccc ttagagctac gcattaaggt tcttgatatc aatgacaacg aaccagtgtt
     1261 cacacaggat gtctttgttg ggtctgttga agagttgagt gcagcacata ctcttgtgat
     1321 gaaaatcaat gcaacagatg cagatgagcc caataccctg aactcgaaaa tttcctatag
     1381 aatcgtatct ctggagcctg cttatcctcc agtgttctac ctaaataaag atacaggaga
     1441 gatttataca accagtgtta ccttggacag agaggaacac agcagctaca ctttgacagt
     1501 agaagcaaga gatggcaatg gagaagttac agacaaacct gtaaaacaag ctcaagttca
     1561 gattcgtatt ttggatgtca atgacaatat acctgtagta gaaaataaag tgcttgaagg
     1621 gatggttgaa gaaaatcaag tcaacgtaga agttacgcgc ataaaagtgt tcgatgcaga
     1681 tgaaataggt tctgataatt ggctggcaaa ttttacattt gcatcaggaa atgaaggagg
     1741 ttatttccac atagaaacag atgctcaaac taacgaagga attgtgaccc ttattaagga
     1801 agtagattat gaagaaatga agaatcttga cttcagtgtt attgtcgcta ataaagcagc
     1861 ttttcacaag tcgattagga gtaaatacaa gcctacaccc attcccatca aggtcaaagt
     1921 gaaaaatgtg aaagaaggca ttcattttaa aagcagcgtc atctcaattt atgttagcga
     1981 gagcatggat agatcaagca aaggccaaat aattggaaat tttcaagcat ttgatgagga
     2041 cactggacta ccagcccatg caagatatgt aaaattagaa gatagagata attggatctc
     2101 tgtggattct gtcacatctg aaattaaact tgcaaaactt cctgattttg aatctagata
     2161 tgttcaaaat ggcacataca ctgtaaagat tgtggccata tcagaagatt atcctagaaa
     2221 aaccatcact ggcacagtcc ttatcaatgt tgaagacatc aacgacaact gtcccacact
     2281 gatagagcct gtgcagacaa tctgtcacga tgcagagtat gtgaatgtta ctgcagagga
     2341 cctggatgga cacccaaaca gtggcccttt cagtttctcc gtcattgaca aaccacctgg
     2401 catggcagaa aaatggaaaa tagcacgcca agaaagtacc agtgtgctgc tgcaacaaag
     2461 tgagaaaaag ctagggagaa gtgaaattca gttcctgatt tcagacaatc agggttttag
     2521 ttgtcctgaa aagcaggtcc ttacactcac agtttgtgag tgtctgcatg gcagcggctg
     2581 cagggaagca cagcatgact cctatgtggg cctgggaccc gcagcaattg cgctcatgat
     2641 tttggccttt ctgctcctgc tattggtgcc acttttactg ctgatgtgcc attgcggaaa
     2701 gggcgccaaa ggctttaccc ccatacctgg caccatagag atgctgcatc cttggaataa
     2761 tgaaggagca ccacctgaag acaaggtggt gccatcattt ctgccagtgg atcaaggggg
     2821 cagtctagta ggaagaaatg gagtaggagg tatggccaag gaagccacga tgaaaggaag
     2881 tagctctgct tccattgtca aagggcaaca tgagatgtcc gagatggatg gaaggtggga
     2941 agaacacaga agcctgcttt ctggtagagc tacccagttt acaggggcca caggcgctat
     3001 catgaccact gaaaccacga agaccgcaag ggccacaggg gcttccagag acatggccgg
     3061 agctcaggca gctgctgttg cactgaacga agagttctta agaaattatt tcactgataa
     3121 agcggcctct tacactgagg aagatgaaaa tcacacagcc aaagattgcc ttctggttta
     3181 ttctcaggaa gaaactgaat cgctgaatgc ttctattggt tgttgcagtt ttattgaagg
     3241 agagctagat gaccgcttct tagatgattt gggacttaaa ttcaagacac tagctgaagt
     3301 ttgcctgggt caaaaaatag atataaataa ggaaattgag cagagacaaa aacctgccac
     3361 agaaacaagt atgaacacag cttcacattc actctgtgag caaactatgg ttaattcaga
     3421 gaatacctac tcctctggca gtagcttccc agttccaaaa tctttgcaag aagccaatgc
     3481 agagaaagta actcaggaaa tagtcactga aaggtctgtg tcttctaggc aggcgcaaaa
     3541 ggtagctaca cctcttcctg acccaatggc ttctagaaat gtgatagcaa cagaaacttc
     3601 ctatgtcaca gggtccacta tgccaccaac cactgtgatc ctgggtccta gccagccaca
     3661 gagccttatt gtgacagaga gggtgtatgc tccagcttct accttggtag atcagcctta
     3721 tgctaatgaa ggtacagttg tggtcactga aagagtaata cagcctcatg ggggtggatc
     3781 gaatcctctg gaaggcactc agcatcttca agatgtacct tacgtcatgg tgagggaaag
     3841 agagagcttc cttgccccca gctcaggtgt gcagcctact ctggccatgc ctaatatagc
     3901 agtaggacag aatgtgacag tgacagaaag agttctagca cctgcttcca ctctgcaatc
     3961 cagttaccag attcccactg aaaattctat gacggctagg aacaccacgg tgtctggagc
     4021 tggagtccct ggccctctgc cagattttgg tttagaggaa tctggtcatt ctaattctac
     4081 cataaccaca tcttccacca gagttaccaa gcatagcact gtacagcatt cttactccta
     4141 aggatccacc ggatctagat aactgatcat aatca
//
