LOCUS       sequence-85280-e        4176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4176)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmoglein2-N-18
FEATURES             Location/Qualifiers
     source          1..4176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3426..3435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3432..4142
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3845..3864
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgtcgcc accatggcgc ggagcccggg acgcgcgtac gccctgctgc
       61 ttctcctgat ctgctttaac gttggaagtg gacttcactt acaggtctta agcacaagaa
      121 atgaaaataa gctgcttcct aaacatcctc atttagtgcg gcaaaagcgc gcctggatca
      181 ccgcccccgt ggctcttcgg gagggagagg atctgtccaa gaagaatcca attgccaaga
      241 tacattctga tcttgcagaa gaaagaggac tcaaaattac ttacaaatac actggaaaag
      301 ggattacaga gccacctttt ggtatatttg tctttaacaa agatactgga gaactgaatg
      361 ttaccagcat tcttgatcga gaagaaacac cattttttct gctaacaggt tacgctttgg
      421 atgcaagagg aaacaatgta gagaaaccct tagagctacg cattaaggtt cttgatatca
      481 atgacaacga accagtgttc acacaggatg tctttgttgg gtctgttgaa gagttgagtg
      541 cagcacatac tcttgtgatg aaaatcaatg caacagatgc agatgagccc aataccctga
      601 actcgaaaat ttcctataga atcgtatctc tggagcctgc ttatcctcca gtgttctacc
      661 taaataaaga tacaggagag atttatacaa ccagtgttac cttggacaga gaggaacaca
      721 gcagctacac tttgacagta gaagcaagag atggcaatgg agaagttaca gacaaacctg
      781 taaaacaagc tcaagttcag attcgtattt tggatgtcaa tgacaatata cctgtagtag
      841 aaaataaagt gcttgaaggg atggttgaag aaaatcaagt caacgtagaa gttacgcgca
      901 taaaagtgtt cgatgcagat gaaataggtt ctgataattg gctggcaaat tttacatttg
      961 catcaggaaa tgaaggaggt tatttccaca tagaaacaga tgctcaaact aacgaaggaa
     1021 ttgtgaccct tattaaggaa gtagattatg aagaaatgaa gaatcttgac ttcagtgtta
     1081 ttgtcgctaa taaagcagct tttcacaagt cgattaggag taaatacaag cctacaccca
     1141 ttcccatcaa ggtcaaagtg aaaaatgtga aagaaggcat tcattttaaa agcagcgtca
     1201 tctcaattta tgttagcgag agcatggata gatcaagcaa aggccaaata attggaaatt
     1261 ttcaagcatt tgatgaggac actggactac cagcccatgc aagatatgta aaattagaag
     1321 atagagataa ttggatctct gtggattctg tcacatctga aattaaactt gcaaaacttc
     1381 ctgattttga atctagatat gttcaaaatg gcacatacac tgtaaagatt gtggccatat
     1441 cagaagatta tcctagaaaa accatcactg gcacagtcct tatcaatgtt gaagacatca
     1501 acgacaactg tcccacactg atagagcctg tgcagacaat ctgtcacgat gcagagtatg
     1561 tgaatgttac tgcagaggac ctggatggac acccaaacag tggccctttc agtttctccg
     1621 tcattgacaa accacctggc atggcagaaa aatggaaaat agcacgccaa gaaagtacca
     1681 gtgtgctgct gcaacaaagt gagaaaaagc tagggagaag tgaaattcag ttcctgattt
     1741 cagacaatca gggttttagt tgtcctgaaa agcaggtcct tacactcaca gtttgtgagt
     1801 gtctgcatgg cagcggctgc agggaagcac agcatgactc ctatgtgggc ctgggacccg
     1861 cagcaattgc gctcatgatt ttggcctttc tgctcctgct attggtgcca cttttactgc
     1921 tgatgtgcca ttgcggaaag ggcgccaaag gctttacccc catacctggc accatagaga
     1981 tgctgcatcc ttggaataat gaaggagcac cacctgaaga caaggtggtg ccatcatttc
     2041 tgccagtgga tcaagggggc agtctagtag gaagaaatgg agtaggaggt atggccaagg
     2101 aagccacgat gaaaggaagt agctctgctt ccattgtcaa agggcaacat gagatgtccg
     2161 agatggatgg aaggtgggaa gaacacagaa gcctgctttc tggtagagct acccagttta
     2221 caggggccac aggcgctatc atgaccactg aaaccacgaa gaccgcaagg gccacagggg
     2281 cttccagaga catggccgga gctcaggcag ctgctgttgc actgaacgaa gagttcttaa
     2341 gaaattattt cactgataaa gcggcctctt acactgagga agatgaaaat cacacagcca
     2401 aagattgcct tctggtttat tctcaggaag aaactgaatc gctgaatgct tctattggtt
     2461 gttgcagttt tattgaagga gagctagatg accgcttctt agatgatttg ggacttaaat
     2521 tcaagacact agctgaagtt tgcctgggtc aaaaaataga tataaataag gaaattgagc
     2581 agagacaaaa acctgccaca gaaacaagta tgaacacagc ttcacattca ctctgtgagc
     2641 aaactatggt taattcagag aatacctact cctctggcag tagcttccca gttccaaaat
     2701 ctttgcaaga agccaatgca gagaaagtaa ctcaggaaat agtcactgaa aggtctgtgt
     2761 cttctaggca ggcgcaaaag gtagctacac ctcttcctga cccaatggct tctagaaatg
     2821 tgatagcaac agaaacttcc tatgtcacag ggtccactat gccaccaacc actgtgatcc
     2881 tgggtcctag ccagccacag agccttattg tgacagagag ggtgtatgct ccagcttcta
     2941 ccttggtaga tcagccttat gctaatgaag gtacagttgt ggtcactgaa agagtaatac
     3001 agcctcatgg gggtggatcg aatcctctgg aaggcactca gcatcttcaa gatgtacctt
     3061 acgtcatggt gagggaaaga gagagcttcc ttgcccccag ctcaggtgtg cagcctactc
     3121 tggccatgcc taatatagca gtaggacaga atgtgacagt gacagaaaga gttctagcac
     3181 ctgcttccac tctgcaatcc agttaccaga ttcccactga aaattctatg acggctagga
     3241 acaccacggt gtctggagct ggagtccctg gccctctgcc agattttggt ttagaggaat
     3301 ctggtcattc taattctacc ataaccacat cttccaccag agttaccaag catagcactg
     3361 tacagcattc ttactccagc agcggtggag caagcgcagc cagtggtagc gcggatccac
     3421 cggtcgccac catggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca
     3481 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     3541 agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg accaagggtg
     3601 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggtct
     3661 acattaagca cccagccgac atccccgact acttcaagct gtccttcccc gagggcttca
     3721 ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac caggactcct
     3781 ccctgcagga cggcgtgttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     3841 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccgaggag cggatgtacc
     3901 ccgaggacgg cgccctgaag agcgagatca agaagaggct gaagctgaag gacggcggcc
     3961 actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     4021 cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac accatcgtgg
     4081 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     4141 aagcggccgc gactctagat cataatcagc catacc
//
