LOCUS       sequence-85281-e        1633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1633)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-EB1-C-18
FEATURES             Location/Qualifiers
     source          1..1633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc
      301 ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac
      361 gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag
      541 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggcagcggtg gaggcagcgc atccggcgga
      781 agcggaagcg cagtgaacgt atactcaacg tcagtgacca gtgataacct aagtcgacat
      841 gacatgctgg cctggatcaa tgagtctctg cagttgaatc tgacaaagat cgaacagttg
      901 tgctcagggg ctgcgtattg tcagtttatg gacatgctgt tccctggctc cattgccttg
      961 aagaaagtga aattccaagc taagctagaa cacgagtaca tccagaactt caaaatatta
     1021 caagcaggtt ttaagagaat gggtgttgac aaaataattc ctgtggacaa attagtaaaa
     1081 ggaaagtttc aggacaattt tgaattcgtt cagtggttca agaagttctt cgatgcaaac
     1141 tatgatggaa aagactatga ccctgtggct gctagacaag gtcaagaaac tgcagtggct
     1201 ccttcccttg ttgctccagc tctgaataaa ccgaagaaac ctctcacttc tagcagtgca
     1261 gctccccaga ggcccatctc aacacagaga accgctgcgg ctcctaaggc tggccctggt
     1321 gtggtgcgaa agaaccctgg tgtgggcaac ggagacgacg aggcagctga gttgatgcag
     1381 caggtcaacg tattgaaact tactgttgaa gacttggaga aagagaggga tttctacttc
     1441 ggaaagctac ggaacattga attgatttgc caggagaacg agggggaaaa cgaccctgta
     1501 ttgcagagga ttgtagacat tctgtatgcc acagatgaag gctttgtgat acctgatgaa
     1561 gggggcccac aggaggagca agaagagtat taaggatcca ccggatctag ataactgatc
     1621 ataatcagcc ata
//
