LOCUS       sequence-85283-e        1684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1684)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-EB3-C-20
FEATURES             Location/Qualifiers
     source          1..1684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     440..459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      241 acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct
      301 tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg
      361 ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg
      481 aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag aggctgaagc
      541 tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg
      601 tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg
      661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      721 acgagctgta caagtccgga ctcagatctg gcagcggtgg aggcagcgca tccggcggaa
      781 gcggaagcgg gggagccgtc aatgtgtact ccacatctgt gaccagtgaa aatctgagtc
      841 gccatgatat gcttgcatgg gtcaacgact ccctgcacct caactatacc aagatagaac
      901 agctttgttc aggggcagcc tactgccagt tcatggacat gctcttcccc ggctgtgtgc
      961 acttgaggaa agtgaagttc caggccaaac tagagcatga atacatccac aacttcaagg
     1021 tgctgcaagc agctttcaag aagatgggtg ttgacaaaat cattcctgta gagaaattag
     1081 tgaaaggaaa attccaagat aattttgagt ttattcagtg gtttaagaaa ttctttgacg
     1141 caaactatga tggaaaggat tacaaccctc tgctggcgcg gcagggccag gacgtagcgc
     1201 cacctcctaa cccaggtgat cagatattca acaaatccaa gaaactcatt ggcacagcag
     1261 ttccacagag gacgtccccc acaggcccaa aaaacatgca gacctctggc cggctgagca
     1321 atgtggcccc cccctgcatt ctccggaaga atcctccatc agcccgaaat ggcggccatg
     1381 agactgatgc ccaaattctt gaactcaacc aacagctggt ggacttgaag ctgacagtgg
     1441 atgggctgga gaaggaacgt gacttctact tcagcaaact tcgtgacatc gagctcatct
     1501 gccaggagca tgaaagtgaa aacagccctg ttatctcagg catcattggc atcctctatg
     1561 ccacagagga aggattcgca ccccctgagg acgatgagat tgaagagcat caacaagaag
     1621 accaggacga gtactaagga tccaccggat ctagataact gatcataatc agccatacca
     1681 catt
//
