LOCUS       sequence-85284-e        1477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1477)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Endo-14
FEATURES             Location/Qualifiers
     source          1..1477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             788..817
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggatc agatctcgag ctcaagcttg atatcgaatt
      781 ccccatggag cagaagctga tctccgagga ggacctgaac cggaagaaac tggtggttgt
      841 tggtgatgga gcctgtggaa agacatgctt gctcatagtc ttcagcaagg acgagttccc
      901 agaggtgtat gtgcccacag tgtttgagaa ctatgtggca gatatcgagg tggatggaaa
      961 gcaggtagag ttggctttgt gggacacagc tggccaggag gactacgacc gcctgcggcc
     1021 gctctcctac ccggacaccg acgtcattct catgtgcttc tcggtggaca gcccggactc
     1081 gctggagaac atccccgaga agtgggtccc cgaggtgaag cacttctgtc ccaatgtgcc
     1141 catcatcctg gtggccaaca aaaaagacct gcgcagcgac gagcatgtcc gcacagagct
     1201 ggcccgcatg aagcaggaac ccgttcgcac ggatgacggc cgcgccatgg ccgtgcgcat
     1261 ccaagcctac gactacctcg agtgctctgc caagaccaag gaaggcgtgc gcgaggtctt
     1321 cgagacggcc acgcgcgccg cgctgcagaa gcgctacggc tcccagaacg gctgcatcaa
     1381 ctgctgcaag gtgctatgag ggccgcgccc gtcgcgcctg cccctgccgg ggatccaccg
     1441 gatctagata actgatcata atcagccata ccacatt
//
