LOCUS       sequence-85285-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     14..34
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..871
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     577..596
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctggtttag tgaaccgtca gatccgctag cgccaccatg ctgctatccg tgccgttgct
      121 gctcggcctc ctcggcctgg ccgtcgccgc accggtcgcc accatggtga gcaagggcga
      181 ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      241 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt
      301 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      361 gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga
      421 ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga
      481 cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa
      541 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      601 gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat
      661 caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
      721 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat
      781 cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      841 ctccaccggc ggcatggacg agctgtacaa gtccggaaag gacgagctgt aagaattctg
      901 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
      961 ataccacatt tgtagaggtt ttacttgctt taaa
//
