LOCUS       sequence-85286-e        2577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions/Membrane, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2577)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Ezrin-C-14
FEATURES             Location/Qualifiers
     source          1..2577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..729
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     435..454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctagcgctac cggtcgccac catggtgagc aagggcgagg agaataacat ggccatcatc
       61 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg
      181 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      241 tccaaggtct acattaagca cccagccgac atccccgact acttcaagct gtccttcccc
      301 gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac
      361 caggactcct ccctgcagga cggcgtgttc atctacaagg tgaagctgcg cggcaccaac
      421 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccgaggag
      481 cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaagaggct gaagctgaag
      541 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
      601 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
      661 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      721 ctgtacaagt ccggactcag atctcgagga agcggcggat ctgggtccgg tccgaaacca
      781 atcaatgtcc gagttaccac catggatgca gagctggagt ttgcaatcca gccaaataca
      841 actggaaaac agctttttga tcaggtggta aagactatcg gcctccggga agtgtggtac
      901 tttggcctcc actatgtgga taataaagga tttcctacct ggctgaagct ggataagaag
      961 gtgtctgccc aggaggtcag gaaggagaat cccctccagt tcaagttccg ggccaagttc
     1021 taccctgaag atgtggctga ggagctcatc caggacatca cccagaaact tttcttcctc
     1081 caagtgaagg aaggaatcct tagcgatgag atctactgcc cccctgagac tgccgtgctc
     1141 ttggggtcct acgctgtgca ggccaagttt ggggactaca acaaagaagt gcacaagtct
     1201 gggtacctca gctctgagcg gctgatccct caaagagtga tggaccagca caaacttacc
     1261 agggaccagt gggaggaccg gatccaggtg tggcatgcgg aacaccgtgg gatgctcaaa
     1321 gataatgcta tgttggaata cctgaagatt gctcaggacc tggaaatgta tggaatcaac
     1381 tatttcgaga taaaaaacaa gaaaggaaca gacctttggc ttggagttga tgcccttgga
     1441 ctgaatattt atgagaaaga tgataagtta accccaaaga ttggctttcc ttggagtgaa
     1501 atcaggaaca tctctttcaa tgacaaaaag tttgtcatta aacccatcga caagaaggca
     1561 cctgactttg tgttttatgc cccacgtctg agaatcaaca agcggatcct gcagctctgc
     1621 atgggcaacc atgagttgta tatgcgccgc aggaagcctg acaccatcga ggtgcagcag
     1681 atgaaggccc aggcccggga ggagaagcat cagaagcagc tggagcggca acagctggaa
     1741 acagagaaga aaaggagaga aaccgtggag agagagaaag agcagatgat gcgcgagaag
     1801 gaggagttga tgctgcggct gcaggactat gaggagaaga caaagaaggc agagagagag
     1861 ctctcggagc agattcagag ggccctgcag ctggaggagg agaggaagcg ggcacaggag
     1921 gaggccgagc gcctagaggc tgaccgtatg gctgcactgc gggctaagga ggagctggag
     1981 agacaggcgg tggatcagat aaagagccag gagcagctgg ctgcggagct tgcagaatac
     2041 actgccaaga ttgccctcct ggaagaggcg cggaggcgca aggaggatga agttgaagag
     2101 tggcagcaca gggccaaaga agcccaggat gacctggtga agaccaagga ggagctgcac
     2161 ctggtgatga cagcaccccc gcccccacca ccccccgtgt acgagccggt gagctaccat
     2221 gtccaggaga gcttgcagga tgagggcgca gagcccacgg gctacagcgc ggagctgtct
     2281 agtgagggca tccgggatga ccgcaatgag gagaagcgca tcactgaggc agagaagaac
     2341 gagcgtgtgc agcggcagct gctgacgctg agcagcgagc tgtcccaggc ccgagatgag
     2401 aataagagga cccacaatga catcatccac aacgagaaca tgaggcaagg ccgggacaag
     2461 tacaagacgc tgcggcagat ccggcagggc aacaccaagc agcgcatcga cgagttcgag
     2521 gccctgtaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctag
//
