LOCUS       sequence-85288-e        4003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal adhesion kinase, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4003)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-FAK-5
FEATURES             Location/Qualifiers
     source          1..4003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     459..478
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gccatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagaata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc
      181 tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc agttcatgta cggctccaag gtctacatta agcacccagc cgacatcccc
      301 gactacttca agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag
      361 gacggcggca ttattcacgt taaccaggac tcctccctgc aggacggcgt gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      481 atgggctggg aggcctccga ggagcggatg taccccgagg acggcgccct gaagagcgag
      541 atcaagaaga ggctgaagct gaaggacggc ggccactacg ccgccgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac
      661 atcgtgtccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctat ggcagcagct
      781 taccttgatc caaacttgaa tcatacacca agttcaagtg caaagacgca cctcggtact
      841 gggatggagc gttccccggg ggccatggag cgagtcctaa aggtttttca ctactttgaa
      901 aacagcagcg agccaacgac gtgggccagc attatccggc atggagatgc tactgatgtt
      961 cgaggcataa tacagaagat tgtggactgt cacaaagtga aaaatgtggc ctgctatggg
     1021 ttgcgactca gtcatctgca gtctgaggag gttcactggc tgcacctgga catgggggta
     1081 tccaatgtga gagagaaatt tgaactagca catcctccag aagaatggaa atatgaactg
     1141 agaattcggt acctgcccaa aggatttcta aaccagttca ctgaggacaa accaacttta
     1201 aattttttct atcagcaggt gaaaaatgac tatatgttag aaatagcaga tcaagtggac
     1261 caggaaattg ctttgaaact aggttgcctt gaaatcagga gatcctacgg agagatgaga
     1321 ggcaatgcat tagagaagaa atccaactat gaagtgctag aaaaagatgt cggtttaaga
     1381 cgattttttc cgaagagttt gctagattca gtgaaggcca aaacactacg aaaattaatc
     1441 caacagacat ttcgacaatt tgccaacctc aacagagaag aaagtatttt gaaattcttt
     1501 gagatcctct ctccagtgta cagatttgac aaggaatgct tcaagtgtgc ccttggttca
     1561 agctggatta tttcagtgga gctggcaatt ggcccagagg aaggaatcag ctaccttaca
     1621 gacaagggtg caaatccaac tcacctggca gattttaatc aagtacaaac tattcagtat
     1681 tcaaacagtg aagacaagga cagaaaaggg atgttgcaac tgaagatagc tggtgcacct
     1741 gagcctctga cagtgacagc accatcctta accattgcag agaatatggc tgacttgata
     1801 gacggatact gccgactggt gaatggagcc acgcaatctt ttattatcag gccacagaaa
     1861 gaaggtgaaa gagctttacc atcaatacca aagctggcca acaatgagaa gcaaggagta
     1921 aggtcgcaca cagtctctgt atcagaaaca gatgactatg cagagataat agatgaagaa
     1981 gatacttata caatgccatc aaccagagat tatgaaattc aaagggagag aattgaactg
     2041 gggcgctgca ttggtgaagg acagtttgga gatgtgcacc aaggaattta catgagtccg
     2101 gaaaatccag ctatggctgt agcaatcaaa acatgtaaaa actgcacctc agacagcgtt
     2161 agagaaaagt tcctacaaga agccttaaca atgcgtcagt ttgatcatcc tcacattgtg
     2221 aagctcattg gagttattac agaaaaccca gtgtggataa tcatggagct ctgtacactt
     2281 ggagagttga gatcgtttct gcaagtaaga aaattcagct tggacctggc ctccctcatc
     2341 ctctacgctt accagcttag cacagcactt gcttacctag agagcaaaag atttgtacat
     2401 agagatattg ctgctaggaa cgtgctggta tctgccactg actgtgtgaa attgggtgac
     2461 tttggcttat cccgatacat ggaagacagt acttactata aagcttccaa aggaaagtta
     2521 cctatcaaat ggatggctcc agagtcaatc aacttccgac ggtttacctc agcaagcgat
     2581 gtgtggatgt ttggtgtgtg tatgtgggag atcctgatgc atggggtaaa gcccttccag
     2641 ggagtgaaaa ataatgatgt tattggtcgg attgagaacg gtgagcggct ccccatgcct
     2701 ccgaactgcc ctcccaccct ctacagcctt atgaccaagt gctgggcata cgaccctagt
     2761 agacgaccca ggtttactga acttaaagca caactcagta caatactgga ggaggagaag
     2821 ctgcagcaag aggaacgaat gagaatggaa tccaggcgac aagtcacagt atcctgggac
     2881 tcaggaggat cagatgaagc tcctcccaag cccagcaggc ctggttaccc cagcccaagg
     2941 tccagtgaag ggttttatcc gagtcctcag catatggtac agccaaatca ctaccaggta
     3001 tctggctact ctggttctca tgggatacca gccatggcag gcagcattta tcctgggcaa
     3061 gcttctctct tggatcaaac agattcctgg aaccatcgac ctcaggaagt atcagcatgg
     3121 cagccaaaca tggaggattc gggcactttg gatgtacgag gaatggggca ggttctgccc
     3181 acacatctca tggaggagag gttaataaga caacagcaag agatggaaga agatcaacgc
     3241 tggcttgaga aagaggaacg attcctggta atgaaacctg atgtgcggct ctccagaggc
     3301 agcattgaac gggaggacgg aggtctccag ggcccagctg gtaaccagca catatatcag
     3361 cctgtgggta aaccagatca tgccgctcca ccaaagaagc cccctcgccc tggagccccc
     3421 cacttgggca gcctcgcgag cctgaacagc cccgtggaca gctacaacga aggcgtgaag
     3481 atcaagccac aggaaatcag ccctcctcct acggccaacc tggaccgctc caatgacaaa
     3541 gtctatgaga atgtaaccgg gctggtgaaa gctgtcatag agatgtccag taaaatacag
     3601 ccagctccgc cagaggagta cgtgcccatg gtaaaggagg ttggcttggc gctgagaacc
     3661 ttgctagcaa cagtggatga gtcgctgcca gtgcttcctg caagcaccca cagagagatt
     3721 gagatggccc agaaactgct gaactctgac ctggctgagc tcattaacaa gatgaagctg
     3781 gcccagcagt acgtcatgac cagcctgcag caggagtaca agaagcaaat gctgacggct
     3841 gctcacgctc tggctgtgga tgccaagaac ttgctggatg tcatcgatca agccagactg
     3901 aaaatgatca gccagtccag gcccactaag ctgccgtagc cgaattctgc agatgggatc
     3961 caccggatct agataactga tcataatcag ccataccaca ttg
//
