LOCUS       sequence-85290-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(1538..1549)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gccatgaagc caggattcag
      781 tccccgtggg ggtggctttg gcggccgagg gggctttggt gaccgtggtg gtcgtggagg
      841 ccgagggggc tttggcgggg gccgaggcca aggcggaggc tttagaggtc gtggacgagg
      901 aggaggtgga ggcggcggcg gcggtggagg aggaggaaga ggtggtggag gcttccattt
      961 tggtggcaac cggggtcgtg gtcggggagg aaaaagagga aaccagtcgg ggaagaatgt
     1021 gatggtggag ccgcatcggc atgagggtgt cttcatttgt cggggaaagg aagatgcact
     1081 ggtcaccaag aacctggtcc ctggggaatc agtttatgga gagaagagag tctcgatttc
     1141 ggaaggagat gacaaaattg agtaccgagc ctggaacccc ttccgctcca agcttgcagc
     1201 agcaatcctg ggtggtgtgg accagatcca catcaaaccg ggggctaagg ttctctacct
     1261 cggggctgcc tcgggcacca cggtctccca tgtctctgac atcgttggtc cggatggtct
     1321 agtctatgca gtcgagttct cccaccgctc tggccgtgac ctcattaact tggccaagaa
     1381 gaggaccaac atcattcctg tgatcgagga tgctcgacac ccacacaaat accgcatgct
     1441 catcgcaatg gtggatgtga tctttgctga tgtggcccag ccagaccaga cccggattgt
     1501 ggccctgaat gcccacacct tcctgcgtaa tggaggacac tttgtgattt ccattaaggc
     1561 caactgcatt gactccacag cctcagccga ggccgtgttt gcctccgaag tgaaaaagat
     1621 gcaacaggag aacatgaagc cgcaggagca gttgaccctt gagccatatg aaagagacca
     1681 tgccgtggtc gtgggagtgt acaggccacc ccccaaggtg aagaactgag gatccaccgg
     1741 atctagataa ctgatcataa tcagccatac cacat
//
