LOCUS       sequence-85292-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-FTR-940
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             193..903
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     606..625
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcaatgctag tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 atgggcatgg cgcgaccacg gggcgcgggg ccctgcagcc ccgggttgga gcgggctccg
      121 cgccggagcg tcggggagct gcgcctgctc ttcgaagcgg gcggatctgg atcggatcca
      181 ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccatcat caaggagttc
      241 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      301 gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt
      361 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggtc
      421 tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc
      481 aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa ccaggactcc
      541 tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      601 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga gcggatgtac
      661 cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa ggacggcggc
      721 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      781 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
      841 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      901 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
      961 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaatgaatgc aattgttgtt
     1021 gttaacttgt ttattgcagc ttataatgtt acaaataaag catag
//
