LOCUS       sequence-85295-e        2181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2181)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..2181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1423..1432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1429..2139
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1842..1861
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgccg agggaaatca tcaccctaca gttgggccag
       61 tgcggcaatc agattgggtt cgagttctgg aaacagctgt gcgccgagca tggtatcagc
      121 cccgagggca tcgtggagga gttcgccacc gagggcactg accacaagga cgtctttttc
      181 taccaggcag acgatgagca ctacatcccc cgggccgtgc tgctggactt ggaaccccgg
      241 gtgatccact ccatcctcaa ctccccctat gccaagctct acaacccaga gaacatctac
      301 ctgtcggaac atggaggagg agctggcaac aactgggcca gcggattctc ccagggagaa
      361 aagatccatg aggacatttt tgacatcata gaccgggagg cagatggtag tgacagtcta
      421 gagggctttg tgctgtgtca ctccattgct ggggggacag gctctggact gggttcctac
      481 ctcttagaac ggctgaatga caggtatcct aagaagctgg tgcagacata ctcagtgttt
      541 cccaaccagg acgagatgag cgatgtggtg gtccagcctt acaattcact cctcacactc
      601 aagaggctga cgcagaatgc agactgtgtg gtggtgctgg acaacacagc cctgaaccgg
      661 attgccacag accgcctgca catccagaac ccatccttct cccagatcaa ccagctggtg
      721 tctaccatca tgtcagccag caccaccacc ctgcgctacc ctggctacat gaacaatgac
      781 ctcatcggcc tcatcgcctc gctcattccc accccacggc tccacttcct catgaccggc
      841 tacacccctc tcactacgga ccagtcagtg gccagcgtga ggaagaccac ggtcctggat
      901 gtcatgaggc ggctgctgca gcccaagaac gtgatggtgt ccacaggccg agaccgccag
      961 accaaccact gctacatcgc catcctcaac atcatccagg gagaggtgga ccccacccag
     1021 gtccacaaga gcttgcagag gatccgggaa cgcaagttgg ccaacttcat cccgtggggc
     1081 cccgccagca tccaggtggc cctgtcgagg aagtctccct acctgccctc ggcccaccgg
     1141 gtcagcgggc tcatgatggc caaccacacc agcatctcct cgctcttcga gagaacctgt
     1201 cgccagtatg acaagctgcg taagcgggag gccttcctgg agcagttccg caaggaggac
     1261 atgttcaagg acaactttga tgagatggac acatccaggg agattgtgca gcagctcatc
     1321 gatgagtacc atgcggccac acggccagac tacatctcct ggggcactca ggagcagcga
     1381 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     1441 ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1501 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1561 gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1621 atcctgtccc ctcagttcat gtacggctcc aaggtctaca ttaagcaccc agccgacatc
     1681 cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc
     1741 gaggacggcg gcattattca cgttaaccag gactcctccc tgcaggacgg cgtgttcatc
     1801 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1861 accatgggct gggaggcctc cgaggagcgg atgtaccccg aggacggcgc cctgaagagc
     1921 gagatcaaga agaggctgaa gctgaaggac ggcggccact acgccgccga ggtcaagacc
     1981 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
     2041 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2101 cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     2161 aatcagccat accacattga g
//
