LOCUS       sequence-85296-e        2114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2114)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-GFAP-N-18
FEATURES             Location/Qualifiers
     source          1..2114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1368..1377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1374..2084
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1787..1806
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggaga ggagacgcat cacctccgct gctcgccgct
       61 cctacgtctc ctcaggggag atgatggtgg ggggcctggc tcctggccgc cgtctgggtc
      121 ctggcacccg cctctccctg gctcgaatgc cccctccact cccaacccgg gtggatttct
      181 ccctggctgg ggcactcaat gctggcttca aggagacccg ggccagtgag cgggcagaga
      241 tgatggagct caatgaccgc tttgccagct acatcgagaa ggttcgcttc ctggaacagc
      301 aaaacaaggc gctggctgct gagctgaacc agctgcgggc caaggagccc accaagctgg
      361 cagacgtcta ccaggctgag ctgcgagagc tgcggctgcg gctcgatcaa ctcaccgcca
      421 acagcgcccg gctggaggtt gagagggaca atctggcaca ggacctggcc actgtgaggc
      481 agaagctcca ggatgaaacc aacctgaggc tggaagccga gaacaacctg gctgcctata
      541 gacaggaagc agatgaagcc accctggccc gtctggatct ggagaggaag attgagtcgc
      601 tggaggagga gatccggttc ttgaggaaga tccacgagga ggaggttcgg gaactccagg
      661 agcagctggc ccgacagcag gtccatgtgg agcttgacgt ggccaagcca gacctcaccg
      721 cagccctgaa agagatccgc acgcagtatg aggcaatggc gtccagcaac atgcatgaag
      781 ccgaagagtg gtaccgctcc aagtttgcag acctgacaga cgctgctgcc cgcaacgcgg
      841 agctgctccg ccaggccaag cacgaagcca acgactaccg gcgccagttg cagtccttga
      901 cctgcgacct ggagtctctg cgcggcacga acgagtccct ggagaggcag atgcgcgagc
      961 aggaggagcg gcacgtgcgg gaggcggcca gttatcagga ggcgctggcg cggctggagg
     1021 aagaggggca gagcctcaag gacgagatgg cccgccactt gcaggagtac caggacctgc
     1081 tcaatgtcaa gctggccctg gacatcgaga tcgccaccta caggaagctg ctagagggcg
     1141 aggagaaccg gatcaccatt cccgtgcaga ccttctccaa cctgcagatt cgagaaacca
     1201 gcctggacac caagtctgtg tcagaaggcc acctcaagag gaacatcgtg gtgaagaccg
     1261 tggagatgcg ggatggagag gtcattaagg agtccaagca ggagcacaag gatgtgatga
     1321 gcagcggtgg agcaagcgca gccagtggta gcgcggatcc accggtcgcc accatggtga
     1381 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     1441 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1501 acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     1561 gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg
     1621 acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga
     1681 acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt
     1741 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1801 agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga
     1861 agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca
     1921 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca
     1981 agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     2041 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgacttcta
     2101 gatcataatc agcc
//
