LOCUS       sequence-85297-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..1011
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     714..733
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcatgaggc ttcgggagcc gctcctgagc
       61 ggcagcgccg cgatgccagg cgcgtcccta cagcgggcct gccgcctgct cgtggccgtc
      121 tgcgctctgc accttggcgt caccctcgtt tactacctgg ctggccgcga cctgagccgc
      181 ctgccccaac tggtcggagt ctccacaccg ctgcagggcg gctcgaacag tgccgccgcc
      241 atcgggcagt cctccgggga gctccggacc ggaggggcca aggatccacc ggtcgccacc
      301 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
      361 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      421 cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      481 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac
      541 ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc
      601 gtgatgaact tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac
      661 ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      721 atgcagaaga agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc
      781 gccctgaaga gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc
      841 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
      901 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa
      961 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg
     1021 actctagatc ata
//
