LOCUS       sequence-85298-e        1224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mApple-H2B-6
FEATURES             Location/Qualifiers
     source          1..1224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1184
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     887..906
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggtga
      481 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      541 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      601 acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      661 gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg
      721 acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga
      781 acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt
      841 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      901 agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga
      961 agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca
     1021 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca
     1081 agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1141 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     1201 atcataatca gccataccac attg
//
