LOCUS       sequence-85300-e        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      501..510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             507..1217
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     920..939
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gccaccatgg
       61 cccgaaccaa gcagaccgct aggaagtcca ccggtgggaa agccccccgc aaacagctgg
      121 ccaccaaggc ggctcggaaa agcgcgccct ctaccggcgg ggtgaagaag cctcaccgct
      181 acaggccagg gaccgtggct ctgagagaga tccgtcgtta ccagaaatcg actgagctgc
      241 tcatccggaa gctgccattc cagagattgg tgagggagat cgcccaggat ttcaaaaccg
      301 acttgaggtt tcaaagtgca gccatcggtg cccttcagga ggctagcgaa gcatacctgg
      361 tggggttgtt tgaagatacc aatctgtgtg ccatccacgc caagagagtc accatcatgc
      421 ccaaagacat ccagttggct cgccggatac ggggggagag agctgggtcc ggaagcgcag
      481 gcagtgggga tccaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggcca
      541 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      601 tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga
      661 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      721 acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct
      781 tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg
      841 ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca
      901 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg
      961 aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag aggctgaagc
     1021 tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg
     1081 tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg
     1141 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     1201 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac cacattt
//
