LOCUS       sequence-85301-e        2471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2471)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Hc-SRC-N-18
FEATURES             Location/Qualifiers
     source          1..2471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1709..1718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1715..2425
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2128..2147
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgtgtaccgt cagatgccgc tagcgctacc ggactcagat ctcgagaccg ccatgggtag
       61 caacaagagc aagcccaagg atgccagcca gcggcgccgc agcctggagc ccgccgagaa
      121 cgtgcacggc gctggcgggg gcgctttccc cgcctcgcag acccccagca agccagcctc
      181 ggccgacggc caccgcggcc ccagcgcggc cttcgccccc gcggccgccg agcccaagct
      241 gttcggaggc ttcaactcct cggacaccgt cacctccccg cagagggcgg gcccgctggc
      301 cggtggagtg accacctttg tggccctcta tgactatgag tctaggacgg agacagacct
      361 gtccttcaag aaaggcgagc ggctccagat tgtcaacaac acagagggag actggtggct
      421 ggcccactcg ctcagcacag gacagacagg ctacatcccc agcaactacg tggcgccctc
      481 cgactccatc caggctgagg agtggtattt tggcaagatc accagacggg agtcagagcg
      541 gttactgctc aatgcagaga acccgagagg gaccttcctc gtgcgagaaa gtgagaccac
      601 gaaaggtgcc tactgcctct cagtgtctga cttcgacaac gccaagggcc tcaacgtgaa
      661 gcactacaag atccgcaagc tggacagcgg cggcttctac atcacctccc gcacccagtt
      721 caacagcctg cagcagctgg tggcctacta ctccaaacac gccgatggcc tgtgccaccg
      781 cctcaccacc gtgtgcccca cgtccaagcc gcagactcag ggcctggcca aggatgcctg
      841 ggagatccct cgggagtcgc tgcggctgga ggtcaagctg ggccagggct gctttggcga
      901 ggtgtggatg gggacctgga acggtaccac cagggtggcc atcaaaaccc tgaagcctgg
      961 cacgatgtct ccagaggcct tcctgcagga ggcccaggtc atgaagaagc tgaggcatga
     1021 gaagctggtg cagttgtatg ctgtggtttc agaggagccc atttacatcg tcacggagta
     1081 catgagcaag gggagtttgc tggactttct caagggggag acaggcaagt acctgcggct
     1141 gcctcagctg gtggacatgg ctgctcagat cgcctcaggc atggcgtacg tggagcggat
     1201 gaactacgtc caccgggacc ttcgtgcagc caacatcctg gtgggagaga acctggtgtg
     1261 caaagtggcc gactttgggc tggctcggct cattgaagac aatgagtaca cggcgcggca
     1321 aggtgccaaa ttccccatca agtggacggc tccagaagct gccctctatg gccgcttcac
     1381 catcaagtcg gacgtgtggt ccttcgggat cctgctgact gagctcacca caaagggacg
     1441 ggtgccctac cctgggatgg tgaaccgcga ggtgctggac caggtggagc ggggctaccg
     1501 gatgccctgc ccgccggagt gtcccgagtc cctgcacgac ctcatgtgcc agtgctggcg
     1561 gaaggagcct gaggagcggc ccaccttcga gtacctgcag gccttcctgg aggactactt
     1621 cacgtccacc gagccccagt accagcccgg ggagaacctc gggcggagta ggggtcgcag
     1681 aggcagcaag cggaagggtg gaccggtcgc caccatggtg agcaagggcg aggagaataa
     1741 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     1801 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
     1861 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     1921 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
     1981 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
     2041 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
     2101 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2161 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
     2221 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
     2281 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
     2341 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     2401 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc aagccatacc
     2461 aacatttgta g
//
