LOCUS       sequence-85303-e        1909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Adaptor, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1909)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Homer1-N-18
FEATURES             Location/Qualifiers
     source          1..1909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1167..1176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1173..1883
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1586..1605
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgccacc atgggggaac aacctatctt cagcactcga gctcatgtct
       61 tccagatcga cccaaacaca aagaagaact gggtacccac cagcaagcat gcagttactg
      121 tgtcttattt ctatgacagc acaaggaatg tgtataggat aatcagttta gacggctcaa
      181 aggcaataat aaatagcacc atcactccaa acatgacatt tactaaaaca tctcaaaagt
      241 ttggccaatg ggctgatagc cgggcaaaca ctgtttatgg actgggattc tcctctgagc
      301 atcatctctc aaaatttgca gaaaagtttc aggaatttaa agaagctgct cggctggcaa
      361 aggagaagtc gcaggagaag atggaactga ccagtacccc ttcacaggaa tcagcaggag
      421 gagatcttca gtctccttta acaccagaaa gtatcaatgg gacagatgat gagagaacac
      481 ccgatgtgac acagaactca gagccaaggg ctgagccagc tcagaatgca ttgccatttt
      541 cacatagtgc cggggatcga acccagggcc tctctcatgc tagttcagcc atcagcaaac
      601 actgggaggc tgaactagcc acgctcaagg ggaacaatgc caagctcacc gcagcgctgc
      661 tggagtccac tgccaacgtg aagcagtgga agcaacagct ggctgcctac caggaggagg
      721 cagagcggct gcacaagcgg gtcacggagc tggaatgtgt tagtagtcaa gcaaacgcgg
      781 tgcacagcca caagacagag ctgagtcaga cagtgcagga gctggaagag accctaaaag
      841 taaaggaaga ggaaatagaa agattaaaac aagaaattga taacgccaga gaacttcaag
      901 aacagaggga ctctttgact cagaaactac aggaagttga gattcgaaat aaagacctgg
      961 aggggcagct gtcggagctg gagcagcgcc tggagaagag ccagagcgag caggacgctt
     1021 tccgcagtaa cctgaagact ctcctagaga ttctggacgg gaaaatattt gaactaacag
     1081 aattgcggga taatttggcc aagctactag aatgcagcgg gtccagcggt tctagcggct
     1141 ccgggagtgg aggggatcca ccggtcgcca ccatggtgag caagggcgag gagaataaca
     1201 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1261 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
     1321 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1381 tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc
     1441 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
     1501 ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc
     1561 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1621 cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc
     1681 tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
     1741 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
     1801 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1861 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatca
//
