LOCUS       sequence-85305-e        2112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Intermediate 
            Filaments, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2112)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1363..2073
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1776..1795
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcac catgagcttc accactcgct ccaccttctc caccaactac
       61 cggtccctgg gctctgtcca ggcgcccagc tacggcgccc ggccggtcag cagcgcggcc
      121 agcgtctatg caggcgctgg gggctctggt tcccggatct ccgtgtcccg ctccaccagc
      181 ttcaggggcg gcatggggtc cgggggcctg gccaccggga tagccggggg tctggcagga
      241 atgggaggca tccagaacga gaaggagacc atgcaaagcc tgaacgaccg cctggcctct
      301 tacctggaca gagtgaggag cctggagacc gagaaccgga ggctggagag caaaatccgg
      361 gagcacttgg agaagaaggg accccaggtc agagactgga gccattactt caagatcatc
      421 gaggacctga gggctcagat cttcgcaaat actgtggaca atgcccgcat cgttctgcag
      481 attgacaatg cccgtcttgc tgctgatgac tttagagtca agtatgagac agagctggcc
      541 atgcgccagt ctgtggagaa cgacatccat gggctccgca aggtcattga tgacaccaat
      601 atcacacgac tgcagctgga gacagagatc gaggctctca aggaggagct gctcttcatg
      661 aagaagaacc acgaagagga agtaaaaggc ctacaagccc agattgccag ctctgggttg
      721 accgtggagg tagatgcccc caaatctcag gacctcgcca agatcatggc agacatccgg
      781 gcccaatatg acgagctggc tcggaagaac cgagaggagc tagacaagta ctggtctcag
      841 cagattgagg agagcaccac agtgctcacc acacagtctg ctgaggttgg agctgctgag
      901 acgacgctca cagagctgag acgtacagtc cagtccttgg agatcgacct ggactccatg
      961 agaaatctga aggccagctt ggagaacagc ctgagggagg tggaggcccg ctacgcccta
     1021 cagatggagc agctcaacgg gatcctgctg caccttgagt cagagctggc acagacccgg
     1081 gcagagggac agcgccaggc ccaggagtat gaggccctgc tgaacatcaa ggtcaagctg
     1141 gaggctgaga tcgccaccta ccgccgcctg ctggaagatg gcgaggactt taatcttggt
     1201 gatgccttgg acagcagcaa ctccatgcaa accatccaaa agaccaccac ccgccggata
     1261 gtggatggca aagtggtgtc tgagaccaat gacaccaaag ttctgaggca tcgaattctg
     1321 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     1381 gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1441 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt
     1501 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1561 tcccctcagt tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac
     1621 tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac
     1681 ggcggcatta ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag
     1741 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1801 ggctgggagg cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc
     1861 aagaagaggc tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac
     1921 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc
     1981 gtgtcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2041 tccaccggcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     2101 ccataccaca tt
//
