LOCUS       sequence-85306-e        2218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytokeratin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2218)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Keratin14-N-18
FEATURES             Location/Qualifiers
     source          1..2218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1481..1490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1487..2197
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1900..1919
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gccaccatga ctacctgcag ccgccagttc acctcctcca gctccatgaa
       61 gggctcctgc ggcatcgggg gcggcatcgg gggcggctcc agccgcatct cctccgtcct
      121 ggccggaggg tcctgccgcg cccccagcac ctacgggggc ggcctgtctg tctcatcctc
      181 ccgcttctcc tctgggggag cctatgggtt ggggggcggc tatggcggtg gcttcagcag
      241 cagcagcagc agctttggta gtggctttgg gggaggatat ggtggtggcc ttggtgctgg
      301 cttgggtggt ggctttggtg gtggctttgc tggtggtgat gggcttctgg tgggcagtga
      361 gaaggtgacc atgcagaacc tcaacgaccg cctggcctcc tacctggaca aggtgcgtgc
      421 tctggaggag gccaacgccg acctggaagt gaagatccgt gactggtacc agaggcagcg
      481 gcctgctgag atcaaagact acagtcccta cttcaagacc attgaggacc tgaggaacaa
      541 gattctcaca gccacagtgg acaatgccaa tgtccttctg cagattgaca atgcccgtct
      601 ggccgcggat gacttccgca ccaagtatga gacagagttg aacctgcgca tgagtgtgga
      661 agccgacatc aatggcctgc gcagggtgct ggacgaactg accctggcca gagctgacct
      721 ggagatgcag attgagagcc tgaaggagga gctggcctac ctgaagaaga accacgagga
      781 ggagatgaat gccctgagag gccaggtggg tggagatgtc aatgtggaga tggacgctgc
      841 acctggcgtg gacctgagcc gcattctgaa cgagatgcgt gaccagtatg agaagatggc
      901 agagaagaac cgcaaggatg ccgaggaatg gttcttcacc aagacagagg agctgaaccg
      961 cgaggtggcc accaacagcg agctggtgca gagcggcaag agcgagatct cggagctccg
     1021 gcgcaccatg cagaacctgg agattgagct gcagtcccag ctcagcatga aagcatccct
     1081 ggagaacagc ctggaggaga ccaaaggtcg ctactgcatg cagctggccc agatccagga
     1141 gatgattggc agcgtggagg agcagctggc ccagctccgc tgcgagatgg agcagcagaa
     1201 ccaggagtac aagatcctgc tggacgtgaa gacgcggctg gagcaggaga tcgccaccta
     1261 ccgccgcctg ctggagggcg aggacgccca cctctcctcc tcccagttct cctctggatc
     1321 gcagtcatcc agagatgtga cctcctccag ccgccaaatc cgcaccaagg tcatggatgt
     1381 gcacgatggc aaggtggtgt ccacccacga gcaggtcctt cgcaccaaga acagcagcgg
     1441 tggagcaagc gcagccagtg gtagcgcgga tccaccggtc gccaccatgg tgagcaaggg
     1501 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     1561 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc
     1621 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     1681 cctgtcccct cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc
     1741 cgactacttc aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga
     1801 ggacggcggc attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta
     1861 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     1921 catgggctgg gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga
     1981 gatcaagaag aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac
     2041 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
     2101 catcgtgtcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     2161 ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcat
//
