LOCUS       sequence-85307-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgatggcgac
      781 tgcgaccccc gtgccgccgc ggatgggcag ccgcgctggc ggccccacca cgccgctgag
      841 ccccacgcgc ctgtcgcggc tccaggagaa ggaggagctg cgcgagctca atgaccggct
      901 ggcggtgtac atcgacaagg tgcgcagcct ggagacggag aacagcgcgc tgcagctgca
      961 ggtgacggag cgcgaggagg tgcgcggccg tgagctcacc ggcctcaagg cgctctacga
     1021 gaccgagctg gccgacgcgc gacgcgcgct cgacgacacg gcccgcgagc gcgccaagct
     1081 gcagatcgag ctgggcaagt gcaaggcgga acacgaccag ctgctcctca actatgctaa
     1141 gaaggaatct gatcttaatg gcgcccagat caagcttcga gaatatgaag cagcactcaa
     1201 ttcgaaagat gcagctcttg ctactgcact tggtgacaaa aaaagtttag agggagattt
     1261 ggaggatctg aaggatcaga ttgcccagtt ggaagcctcc ttagctgcag ccaaaaaaca
     1321 gttagcagat gaaactttac ttaaagtaga tttggagaat cgttgtcaga gccttactga
     1381 ggacttggag tttcgcaaaa gcatgtatga agaggagatt aacgagacca gaaggaagca
     1441 tgaaacgcgc ttggtagagg tggattctgg gcgtcaaatt gagtatgagt acaagctggc
     1501 gcaagccctt catgagatga gagagcaaca tgatgcccaa gtgaggctgt ataaggagga
     1561 gctggagcag acttaccatg ccaaacttga gaatgccaga ctgtcatcag agatgaatac
     1621 ttctactgtc aacagtgcca gggaagaact gatggaaagc cgcatgagaa ttgagagcct
     1681 ttcatcccag ctttctaatc tacagaaaga gtctagagca tgtttggaaa ggattcaaga
     1741 attagaggac ttgcttgcta aagaaaaaga caactctcgt cgcatgctga cagacaaaga
     1801 gagagagatg gcggaaataa gggatcaaat gcagcaacag ctgaatgact atgaacagct
     1861 tcttgatgta aagttagccc tggacatgga aatcagtgct tacaggaaac tcttagaagg
     1921 cgaagaagag aggttgaagc tgtctccaag cccttcttcc cgtgtgacag tatcccgagc
     1981 atcctcaagt cgtagtgtac gtacaactag aggaaagcgg aagagggttg atgtggaaga
     2041 atcagaggcg agtagtagtg ttagcatctc tcattccgcc tcagccactg gaaatgtttg
     2101 catcgaagaa attgatgttg atgggaaatt tatccgcttg aagaacactt ctgaacagga
     2161 tcaaccaatg ggaggctggg agatgatcag aaaaattgga gacacatcag tcagttataa
     2221 atatacctca agatatgtgc tgaaggcagg ccagactgtt acaatttggg ctgcaaacgc
     2281 tggtgtcaca gccagccccc caactgacct catctggaag aaccagaact cgtggggcac
     2341 tggcgaagat gtgaaggtta tattgaaaaa ttctcaggga gaggaggttg ctcaaagaag
     2401 tacagtcttt aaaacaacca tacctgaaga agaggaggag gaggaagaag cagctggagt
     2461 ggttgttgag gaagaacttt tccaccagca gggaacccca agagcatcca atagaagctg
     2521 tgcaattatg tagggatcca ccggatctag ataact
//
