LOCUS       sequence-85311-e        2059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2059)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..110,111..1268)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1329..2039
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1742..1761
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acggtcagat ccgctagcgc taccggactc agatctcgag cgccaccatg gcggccccgg
       61 gcgcccggcg tccgctgctc ctgttgctgc tggcaggcct tgcacacagc gccccagcac
      121 tgttcgaggt gaaagacaac aacggcacag cgtgtataat ggccagcttc tctgcctcct
      181 ttctgaccac ctatgaggct ggacatgttt ctaaggtctc gaatatgacc ctgccagcct
      241 ctgcagaagt cctgaagaat agcagctctt gtggtgaaaa gaatgcttct gagcccaccc
      301 tcgcaatcac ctttggagaa ggatatttac tgaaactcac cttcacaaaa aacacaacac
      361 gttacagtgt ccagcacatg tatttcacat ataacctgtc agacacacaa ttctttccca
      421 atgccagctc caaagggccc gacactgtgg attccacaac tgacatcaag gcagacatca
      481 acaaaacata ccgatgtgtc agcgacatca gggtctacat gaagaatgtg accattgtgc
      541 tctgggacgc tactatccag gcctacctgc cgagtagcaa cttcagcaag gaagagacac
      601 gctgcccaca ggatcaacct tccccaacta ctgggccacc cagcccctca ccaccacttg
      661 tgcccacaaa ccccagtgtg tccaagtaca atgtgactgg tgacaatgga acctgcctgc
      721 tggcctctat ggcactgcaa ctcaacatca cctacatgaa gaaggacaac acgactgtga
      781 ccagagcatt caacatcaac ccaagtgaca aatatagtgg gacttgcggt gcccagttgg
      841 tgaccctgaa ggtggggaac aagagcagag tcctggagct gcagtttggg atgaatgcca
      901 cttctagcct gtttttcctg caaggagttc agttgaacat gactcttcct gatgccatag
      961 agcccacgtt cagcacctcc aactattccc tgaaagctct tcaggccagt gtcggcaact
     1021 catacaagtg caacagtgag gagcacatct ttgtcagcaa ggcgctcgcc ctcaatgtct
     1081 tcagcgtgca agtccaggct ttcagggtag aaagtgacag gtttgggtct gtggaagagt
     1141 gtgtacagga cggtaacaac atgctgatcc ccattgctgt gggcggggcc ctggcagggc
     1201 tggtcctcat cgtcctcatc gcctacctca tcggcaggaa gaggagtcac gcgggctatc
     1261 agaccatctc ggaattcggc tccaccggct ccaccggctc caccggcgcg gatccaccgg
     1321 tcgccaccat ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc
     1381 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1441 gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc aagggtggcc
     1501 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggtctaca
     1561 ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaggt
     1621 gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag gactcctccc
     1681 tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     1741 gccccgtaat gcagaagaag accatgggct gggaggcctc cgaggagcgg atgtaccccg
     1801 aggacggcgc cctgaagagc gagatcaaga agaggctgaa gctgaaggac ggcggccact
     1861 acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     1921 acatcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc atcgtggaac
     1981 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
     2041 cggccgcgac tctagatca
//
