LOCUS       sequence-85312-e        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      377..386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             383..1093
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     796..815
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gccaccatga agttaagtcg ccagttcacc gtgtttggca gcgcgatctt
       61 ctgcgtcgta atcttctcac tctacctgat gctggacagg ggtcacttgg actaccctcg
      121 gggcccgcgc caggagggct cctttccgca gggccagctt tcaatattgc aagaaaagat
      181 tgaccatttg gagcgtttgc tcgctgagaa caacgagatc atctcaaata tcagagactc
      241 agtcatcaac ctgagcgagt ctgtggagga cggcccgcgg gggtcaccag gcaacgccag
      301 ccaaggctcc atccacctcc actcgccaca gttggccctg caggctgacc ccagctccgg
      361 atcggatcca ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccatcat
      421 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      481 gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt
      541 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      601 ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
      661 cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa
      721 ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa
      781 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga
      841 gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa
      901 ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
      961 gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta
     1021 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     1081 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca tt
//
