LOCUS       sequence-85313-e        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc
      301 ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac
      361 gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag
      541 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggtggctccg caggatcccg gcaagaagaa
      781 gcaaaggctg ctgtaggtgt gactggaaat gacatcacta ccccgccaaa caaggagcca
      841 ccaccaagcc cagaaaagaa agcaaagcct ttggccacca ctcaacctgc aaagacttca
      901 acatcgaaag ccaaaacaca gcccacttct ctccctaagc aaccagctcc caccacctct
      961 ggtgggttga ataaaaaacc catgagcctc gcctcaggct cagtgccagc tgccccacac
     1021 aaacgccctg ctgctgccac tgctactgcc aggccttcca ccctacctgc cagagacgtg
     1081 aagccaaagc caattacaga agctaaggtt gccgaaaagc ggacctctcc atccaagcct
     1141 tcatctgccc cagccctcaa acctggacct aaaaccaccc caaccgtttc aaaagccaca
     1201 tctccctcaa ctcttgtttc cactggacca agtagtagaa gtccagctac aactctgcct
     1261 aagaggccaa ccagcatcaa gactgagggg aaacctgctg atgtcaaaag gatgactgct
     1321 aagtctgcct cagctgactt gagtcgctca aagaccacct ctgccagttc tgtgaagaga
     1381 aacaccactc ccactggggc agcaccccca gcagggatga cttccactcg agtcaagccc
     1441 atgtctgcac ctagccgctc ttctggggct ctttctgtgg acaagaagcc cacttccact
     1501 aagcctagct cctctgctcc cagggtgagc cgcctggcca caactgtttc tgcccctgac
     1561 ctgaagagtg ttcgctccaa ggtcggctct acagaaaaca tcaaacacca gcctggagga
     1621 ggccgggcca aggtagagaa aaaaacagag gcagctacca cagctgggaa gcctgaacct
     1681 aatgcagtca ctaaagcagc cggctccatt gcgagtgcac agaaaccgcc tgctgggaaa
     1741 gtccagatag tatccaaaaa agtgagctac agtcatattc aatccaagtg tgtttccaag
     1801 gacaatatta agcatgtccc tggatgtggc aatgttcaga ttcagaacaa gaaagtggac
     1861 atatccaagg tctcctccaa gtgtgggtcc aaagctaata tcaagcacaa gcctggtgga
     1921 ggagatgtca agattgaaag tcagaagttg aacttcaagg agaaggccca agccaaagtg
     1981 ggatagggat ccaccggatc tagataactg atcataatca gccataccac attt
//
