LOCUS       sequence-85315-e        1860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1860)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1104..1113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1110..1820
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1523..1542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cggactcaga tctcgaggcc accatggctg agccccgcca ggagttcgaa gtgatggaag
       61 atcacgctgg gacgtacggg ttgggggaca ggaaagatca ggggggctac accatgcacc
      121 aagaccaaga gggtgacacg gacgctggcc tgaaagctga agaagcaggc attggagaca
      181 cccccagcct ggaagacgaa gctgctggtc acgtgaccca agctcgcatg gtcagtaaaa
      241 gcaaagacgg gactggaagc gatgacaaaa aagccaaggg ggctgatggt aaaacgaaga
      301 tcgccacacc gcggggagca gcccctccag gccagaaggg ccaggccaac gccaccagga
      361 ttccagcaaa aaccccgccc gctccaaaga caccacccag ctctggtgaa cctccaaaat
      421 caggggatcg cagcggctac agcagccccg gctccccagg cactcccggc agccgctccc
      481 gcaccccgtc ccttccaacc ccacccaccc gggagcccaa gaaggtggca gtggtccgta
      541 ctccacccaa gtcgccgtct tccgccaaga gccgcctgca gacagccccc gtgcccatgc
      601 cagacctgaa gaatgtcaag tccaagatcg gctccactga gaacctgaag caccagccgg
      661 gaggcgggaa ggtgcaaata gtctacaaac cagttgacct gagcaaggtg acctccaagt
      721 gtggctcatt aggcaacatc catcataaac caggaggtgg ccaggtggaa gtaaaatctg
      781 agaagcttga cttcaaggac agagtccagt cgaagattgg gtccctggac aatatcaccc
      841 acgtccctgg cggaggaaat aaaaagattg aaacccacaa gctgaccttc cgcgagaacg
      901 ccaaagccaa gacagaccac ggggcggaga tcgtgtacaa gtcgccagtg gtgtctgggg
      961 acacgtctcc acggcatctc agcaatgtct cctccaccgg cagcatcgac atggtagact
     1021 cgccccagct cgccacgcta gctgacgagg tgtctgcctc cctggccaag cagggtttga
     1081 gctccggagg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag aataacatgg
     1141 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1201 agttcgagat cgagggcgag ggcgagggcc gcccctacga ggcctttcag accgctaagc
     1261 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1321 tgtacggctc caaggtctac attaagcacc cagccgacat ccccgactac ttcaagctgt
     1381 ccttccccga gggcttcagg tgggagcgcg tgatgaactt cgaggacggc ggcattattc
     1441 acgttaacca ggactcctcc ctgcaggacg gcgtgttcat ctacaaggtg aagctgcgcg
     1501 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1561 ccgaggagcg gatgtacccc gaggacggcg ccctgaagag cgagatcaag aagaggctga
     1621 agctgaagga cggcggccac tacgccgccg aggtcaagac cacctacaag gccaagaagc
     1681 ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcgtg tcccacaacg
     1741 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1801 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacattt
//
