LOCUS       sequence-85316-e        1862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1862)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1107..1116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1113..1823
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1526..1545
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggatc tgcaagcctc gttgctgtcc actggcccca
       61 atgccagcaa catctccgat ggccaggata atttcacatt ggcggggcca cctcctcgca
      121 caaggagtgt ctcctacatc aacatcatca tgccttcagt gtttggtacc atctgtctcc
      181 tgggcattgt gggaaactcc acagtcattt ttgccgtggt gaagaaatcc aagctgcact
      241 ggtgcagcaa cgtccctgac atcttcatca tcaacctctc tgtggtggat ctgcttttcc
      301 tgctgggcat gcctttcatg atccaccagc tcatgggtaa tggtgtctgg cactttgggg
      361 aaaccatgtg caccctcatc acagccatgg acgccaacag tcagttcacc agcacctaca
      421 tcctgactgc tatggccatt gaccgctact tggccaccgt ccatcccatc tcctccacca
      481 agttccggaa gccctccatg gccaccctgg tgatctgcct cctgtgggct ctctcgttca
      541 ttagcatcac tcctgtgtgg ctctatgcca ggcttatccc cttcccaggg ggtgctgtgg
      601 gctgtggcat ccgcctacca aacccagata ctgatcttta ctggttcact ctgtatcagt
      661 ttttcctggc cttcgccctt ccgtttgtgg tcatcactgc tgcgtacgtg aaaatactac
      721 agcgcatgac gtcttcggtg gccccagcct ctcaacgcag catccggctt cggacaaaga
      781 gggtgacccg cacagccatt gccatctgtc tggtcttctt tgtgtgctgg gcgccctact
      841 acgtgctgca gctgacccag ttgtccatca gccgcccgac cctcacattc gtctacctgt
      901 acaatgcggc tatcagcttg ggctatgcca acagctgcct caatcccttt gtgtacatag
      961 tactctgtga gacctttcga aaacgcttgg tgctgtcggt gaagcccgcg gcccaggggc
     1021 agcttcgcac ggtcagcaat gctcagacag ctgacgagga gaggacagaa agcaaaggca
     1081 ccggaggtag cggggatcca ccggtcgcca ccatggtgag caagggcgag gagaataaca
     1141 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1201 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
     1261 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1321 tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc
     1381 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
     1441 ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc
     1501 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1561 cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc
     1621 tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
     1681 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
     1741 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1801 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     1861 tt
//
