LOCUS       sequence-85317-e        6756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der 
            Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, 
            Lippincott-Schwartz J
  TITLE     A contractile and counterbalancing adhesion system controls the 3D 
            shape of crawling cells.
  JOURNAL   J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. 
            Epub 2014 Apr 7.
  PUBMED    24711500
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201311104"; journalName: "J Cell Biol"; date: 
            "2014-04-14- 14"; volume: "205"; issue: "1"; pages: "83-96"
COMMENT     Sequence Label: mApple-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..745
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     451..470
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagaa
       61 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac
      181 cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      241 tcagttcatg tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt
      301 caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg
      361 cattattcac gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa
      421 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      481 ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa
      541 gaggctgaag ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc
      601 caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc
      661 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      721 cggcggcatg gacgagctgt acaagtccgg actcagatct ggcagcggtg gaggcagcgc
      781 atccggcgga agcggaagcg ctcagcaggc tgcagacaag tacctctatg tggataaaaa
      841 cttcatcaat aacccgctgg cccaagctga ctgggctgcc aagaagttgg tatgggtgcc
      901 ttccagcaag aatggctttg aaccagccag cctcaaggag gaggtgggag aagaggccat
      961 tgtagagctg gtagagaatg ggaagaaggt gaaggtgaac aaggacgaca tccagaagat
     1021 gaacccaccc aagttctcca aggtggagga catggcagag ctcacgtgcc tcaacgaagc
     1081 ttcggtgctg cacaacctca aggagcgata ctactcaggg cttatctaca cctattcagg
     1141 cctgttctgt gtggtcatca acccttataa gaacctgccc atctactcag aggagatcgt
     1201 ggagatgtac aagggcaaga agaggcacga gatgccaccc cacatctacg ccatcacaga
     1261 tactgcctac cggagcatga tgcaggaccg ggaagatcag tccatcctgt gcacggggga
     1321 gtctggagca gggaagacag agaacaccaa gaaagtcatc cagtacctgg cacatgtggc
     1381 ctcctcacac aagagcaaga aggaccaggg ggagttggag cggcagctgc tacaggccaa
     1441 ccctatccta gaggcctttg gaaacgccaa gacggtgaag aatgacaact cctctcgatt
     1501 cggtaaattc attcgtatca actttgatgt caatggctac attgttggtg ccaacattga
     1561 gacttatctt ctggagaaat ctcgtgctat ccgccaagcc aaagaggagc ggaccttcca
     1621 catcttctac tacctgctgt ctggggccgg agaacacctg aagactgatc tcctgttgga
     1681 gccatacaac aaataccgct tcctgtccaa cgggcacgtc accatccctg ggcagcagga
     1741 caaggacatg ttccaggaga caatggaggc catgagaatt atgggtatcc cagaggatga
     1801 gcagatgggc ttgctgcggg tcatctctgg ggtccttcag cttggcaaca ttgccttcaa
     1861 gaaggagcgg aacactgacc aggcgtccat gccggacaac acagctgctc aaaaggtgtc
     1921 ccacctcctg gggatcaatg tgaccgactt caccagaggc atcctcaccc cacgcatcaa
     1981 ggtgggcaga gactatgtgc agaaggcgca gactaaagag caggctgact ttgccattga
     2041 ggccttggcc aaggctacct atgagcggat gttccgctgg ctggtgcttc gcatcaacaa
     2101 agctctggac aagaccaaga ggcagggcgc ctcatttatc gggatactgg acatcgctgg
     2161 ctttgaaatc tttgatctga actccttcga gcagctgtgc atcaactaca ccaacgagaa
     2221 gctgcagcag ctgttcaacc acaccatgtt catcctggag caggaggagt accagcgaga
     2281 gggcatcgag tggaacttca tcgacttcgg cctggacctg cagccctgca tcgacctcat
     2341 tgagaagccg gcgggtcccc caggcatcct ggccctgcta gatgaggagt gctggtttcc
     2401 taaggccact gacaagagct tcgtggagaa ggtggtgcag gagcagggca cccaccccaa
     2461 gttccagaag cccaagcaac tgaaggacaa ggctgatttc tgcattatcc actatgccgg
     2521 caaggtggac tataaagctg acgagtggct gatgaagaac atggacccct tgaacgacaa
     2581 catcgccacg ctgcttcacc agtcctcaga caagtttgtc tctgagctgt ggaaggatgt
     2641 ggatcggatc attggcttgg accaagtggc tggaatgtcc gagacagcac tacctggtgc
     2701 cttcaagacc cggaagggca tgttccgtac tgtcggacag ctgtacaagg agcagctggc
     2761 caagctcatg gccacgttga ggaataccaa ccccaacttc gtgcgctgca tcattcccaa
     2821 ccatgagaag aaggccggca aactggaccc gcacttggtg ctggaccagc tgcgctgcaa
     2881 tggcgtcctt gagggcatcc ggatctgccg ccagggcttt cccaacaggg tggtcttcca
     2941 ggagttccgg cagaggtatg agatcctcac ccccaactcc atcccgaagg gcttcatgga
     3001 tggcaagcaa gcgtgtgtgc tcatgatcaa agccttggag cttgacagca acctgtaccg
     3061 catcggccag agcaaagtgt tcttccgggc aggagtgctc gcccacctgg aggaagagcg
     3121 ggacctgaag atcaccgatg tcatcattgg cttccaggcc tgctgcaggg gctacctggc
     3181 caggaaggcc tttgccaaga ggcagcaaca gctgaccgcc atgaaggtcc tacagaggaa
     3241 ctgtgctgcg tacctcaggc tgcgcaactg gcagtggtgg aggctcttca ccaaggtcaa
     3301 gcccctgttg aactcaataa gacatgagga tgagctgtta gccaaggagg cggaactgac
     3361 aaaggttcga gagaaacatc tggctgcaga gaacaggctg acagagatgg agacgatgca
     3421 gtctcagctc atggcagaga agctgcagct tcaggagcag ctgcaggcgg agacagagct
     3481 gtgtgccgag gctgaggagc tccgggcccg tctgacagcg aagaagcagg agctggagga
     3541 gatatgccat gacctggagg ccagggtgga ggaggaggag gagcgctgcc agtacctgca
     3601 ggccgagaag aagaagatgc agcagaacat ccaggaactt gaggagcagt tggaggagga
     3661 ggagagcgcc cggcagaagc tgcagcttga gaaggtgacc accgaggcca agctgaagaa
     3721 actggaggag gaccagatca tcatggagga ccagaactgc aaactggcca aggagaagaa
     3781 actgctggaa gacagagtag ctgagttcac taccaacctc atggaagagg aggagaagtc
     3841 caagagcctg gccaagctca agaacaagca cgaggcaatg atcaccgacc tggaagagcg
     3901 cctccgtagg gaggagaagc agaggcagga gttggagaag acccgtcgca agctggaggg
     3961 agactccaca gacctcagtg accagattgc tgagctccag gcgcagatag cagagctcaa
     4021 gatgcagctg gccaagaagg aggaggagtt gcaggctgcc ttggccagag tggaagaaga
     4081 agctgctcag aagaatatgg ccctgaagaa gatccgagaa ctggaaactc agatatctga
     4141 gctccaggag gacctggagt cggagcgagc ctccaggaat aaagccgaga agcagaaacg
     4201 ggatctggga gaggagctgg aggcgctgaa gacagagctg gaggacacgc tggactccac
     4261 ggctgcccag caggagctga ggtcgaagcg tgagcaggag gtgagcatcc tgaagaagac
     4321 tctggaggac gaggccaaga cccatgaggc ccagatccag gagatgaggc agaagcactc
     4381 acaggctgtg gaggagctgg cagatcagtt ggagcagacg aagcgggtaa aagctaccct
     4441 tgagaaggcg aagcagaccc tggagaatga gcggggagag ctggccaatg aggtgaaggc
     4501 cctgctgcaa ggcaagggcg actcagagca caagcgcaag aaggtggagg cgcagctgca
     4561 agaactgcag gtcaagttca gcgagggaga gcgcgtgcga accgaactgg ccgacaaggt
     4621 caccaagctg caggttgaac tggacagtgt gaccggtctc cttagccagt ctgacagcaa
     4681 gtccagcaag cttacgaagg acttctctgc gctggagtcc cagcttcagg acacacagga
     4741 gttgctccag gaggagaacc ggcagaagct gagcctgagc accaagctca agcagatgga
     4801 ggatgagaaa aactccttca gggagcagct ggaggaggag gaggaggcca agcgcaactt
     4861 ggagaagcag atcgccacgc tccatgccca ggtgaccgac atgaagaaga agatggagga
     4921 cggtgtaggg tgcctggaga ctgcagagga ggcgaagcgg aggcttcaga aggacttgga
     4981 aggcctgagc cagcggcttg aggagaaggt ggctgcctac gataagctgg agaagaccaa
     5041 gacacggctg cagcaggagc tggacgacct gctggttgac ctggaccacc agcggcagag
     5101 cgtctccaac ctggaaaaga agcagaagaa gttcgaccag ctcctagccg aggagaagac
     5161 catctcggcc aagtatgcag aggagcgtga ccgagctgag gctgaggccc gtgagaagga
     5221 gacaaaggcg ctatcactgg cccgggcgct tgaggaggcc atggagcaga aggcagagct
     5281 ggagcggctc aacaagcagt tccgcacgga gatggaggac ctcatgagct ccaaggatga
     5341 cgtgggcaag agtgtccacg agctggagaa gtccaagcgg gccttggagc agcaggtgga
     5401 ggagatgaag acccagctgg aggagctgga ggatgagctg caggccacgg aggatgccaa
     5461 gctccgcctg gaggtgaacc tgcaggccat gaaggcccag tttgagcggg atctgcaggg
     5521 ccgggatgaa cagagcgagg agaagaagaa gcagctggtc agacaggtgc gggagatgga
     5581 ggcggagctg gaggatgaga ggaagcagcg ctccatggcc atggccgcac gcaagaaact
     5641 ggagatggat ctgaaggacc tggaggcaca cattgacaca gccaataaga accgggaaga
     5701 ggccatcaaa cagctgcgga agcttcaggc ccagatgaag gactgcatgc gggagctgga
     5761 cgacacgcgc gcctcccggg aggagatcct ggcgcaggcc aaggagaatg agaagaagct
     5821 gaagagcatg gaggccgaga tgattcagct gcaggaggaa ctggcagctg ctgagcgtgc
     5881 taagcgtcag gcccaacagg aacgggacga gctggctgat gagatcgcca acagcagtgg
     5941 caaaggggcc ctagcattag aggagaagcg gcgactggag gcccgcattg ccctgctgga
     6001 ggaggagctg gaggaggaac agggcaacac ggagctgatc aacgatcggc tgaagaaggc
     6061 caacctgcag atcgaccaaa taaacaccga cctgaacctg gaacgcagcc acgcacagaa
     6121 gaatgagaat gcgcgacagc agctggaacg ccagaacaag gagctcaagg ccaagctgca
     6181 ggaaatggag agtgctgtca agtccaaata caaggcctcc atcgcggcct tggaggccaa
     6241 aattgcacag ctggaggaac agctggacaa cgagaccaag gagcgccagg cagcctccaa
     6301 gcaggtgcgc cggacggaga agaagctgaa ggacgtgctg ctgcaggtgg aggacgagcg
     6361 gaggaacgcg gaacagttca aggaccaggc tgacaaggcg tccacccgcc tgaagcagct
     6421 taaacggcag ctagaggagg ctgaagagga ggcccagcgg gccaatgcct cacgccggaa
     6481 gctgcagcgt gagctggaag atgccacaga gaccgctgat gctatgaacc gcgaggtcag
     6541 ctccctgaag aacaaactga ggcgtgggga cctgccattt gtcgtgactc gccgaattgt
     6601 tcggaaaggc actggcgact gctcagacga ggaggtcgac ggtaaagcag atggggccga
     6661 tgccaaggca gctgaataga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     6721 atctagataa ctgatcataa tcagccatac cacatt
//
