LOCUS       sequence-85321-e        2326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2326)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..743
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     449..468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagaata
       61 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      121 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg
      181 ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      241 agttcatgta cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca
      301 agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca
      361 ttattcacgt taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc
      421 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      481 aggcctccga ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga
      541 ggctgaagct gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca
      601 agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc
      661 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      721 gcggcatgga cgagctgtac aagtccggaa gtgctgggag cgcaagcgga agcggaggct
      781 ccggttttaa ctacgaacgt ccaaaacact tcatccagtc ccaaaaccca tgtggctcca
      841 gattgcagcc tcctggacca gaaacctcca gcttctctag ccagaccaaa cagtcttcca
      901 ttatcatcca gccccgccag tgtacagagc aaagattttc tgcctcctca acactgagct
      961 ctcacatcac catgtcctcc tctgctttcc ctgcttctcc ccagcagcat gctggctcca
     1021 acccaggcca aagggttaca accacctata accagtcccc agccagcttc ctcagctcca
     1081 tattaccatc acagcctgat tacaatagca gtaaaatccc ttccgctatg gattccaact
     1141 atcaacagtc ctcagctggc caacctataa atgcaaagcc atcccaaact gcaaatgcta
     1201 agcccatacc aagaactcct gatcatgaaa tacaaggatc aaaagaagct ttgattcaag
     1261 atttggaaag aaagctgaaa tgcaaggaca cccttcttca taatggaaat caacgtctaa
     1321 catatgaaga gaagatggct cgcagattgc taggaccaca gaatgcagct gctgtgtttc
     1381 aagctcagga tgacagtggt gcacaagact cgcagcaaca caactcagaa catgcgcgac
     1441 tgcaagttcc tacatcacaa gtaagaagta gatcaacctc aaggggagat gtgaatgatc
     1501 aggatgcaat ccaggagaaa ttttacccac cacgtttcat tcaagtgcca gagaacatgt
     1561 cgattgatga aggaagattc tgcagaatgg acttcaaagt gagtggactg ccagctcctg
     1621 atgtgtcatg gtatctaaat ggaagaacag ttcaatcaga tgatttgcac aaaatgatag
     1681 tgtctgagaa gggtcttcat tcactcatct ttgaagtagt cagagcttca gatgcagggg
     1741 cttatgcatg tgttgccaag aatagagcag gagaagccac cttcactgtg cagctggatg
     1801 tccttgcaaa agaacataaa agagcaccaa tgtttatcta caaaccacag agcaaaaaag
     1861 ttttagaggg agattcagtg aaactagaat gccagatctc ggctatacct ccaccaaagc
     1921 ttttctggaa aagaaataat gaaatggtac aattcaacac tgaccgaata agcttatatc
     1981 aagataacac tggaagagtt actttactga taaaagatgt aaacaagaaa gatgctgggt
     2041 ggtatactgt gtcagcagtt aatgaagctg gagtgactac atgtaacaca agattagacg
     2101 ttacggcacg tccaaaccaa actcttccag ctcctaagca gttacgggtt cgaccaacat
     2161 tcagcaaata tttagcactt aatgggaaag gtttgaatgt aaaacaagct tttaacccag
     2221 aaggagaatt tcagcgtttg gcagctcaat ctggactcta tgaaagtgaa gaactttaag
     2281 gatccaccgg atctagataa ctgatcataa tcagccatac cacatt
//
