LOCUS       sequence-85322-e        2829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2829)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag tgagcgctct ggcctcccgc
      781 gctaccgcca tgcagtcctc cggctccttc aactacgcgc gccctaagca gttcatcgcg
      841 gcgcagaacc tgggtcccgc atcggggctg cccacgccaa cctccagccc cagctcctcc
      901 agcttgccgt caccattgtc ccccacgccc aggcccttcg gccgcgcgcc cgggccgccc
      961 ttcgtggaac ccgaggccat gtggggcccg tcctcgccct cgccgccacc gccgccacct
     1021 ccggtcttca gcccctctgc agcctacccg gtgcccgacg tgttcccact gccaccgccg
     1081 ccgccaccgc tacctagctc cacctcacac tgcgcctcgc ccgcccgctt cggccccagc
     1141 cagacgcccg cagccttcct cagcgccctg ctgccctctc agccaccgcc tgtcgctgtc
     1201 aacgccctgg ggctgcccaa gggagtcacc cccgcggggt tcccaaagaa gtccagtaga
     1261 actgctagaa ttgcctctga tgaggagatt caaggcacaa aggatgctgt catccaagac
     1321 ctggaacgga agcttcgctt caaggaggac cttctgaaca atggccaacc gaggctaacc
     1381 tatgaggaaa gaatggctcg ccgcctgctt ggagccgaca gcgcaaacgt cttcaacatc
     1441 caggagccag aggaaacggc agccaatcag gagtacaaag tctctagctg cgagcagagg
     1501 ctgattagcg agattgaata caggctggag cgctcccctg tggatgagtc gggagacgag
     1561 gtgcaggatc cagatgtgcc tgtggagaac gcaacagctc ccttctttga gatgaagctg
     1621 aaacactaca agatctttga ggggatgccg gtgactttca cgtgtcgagt ggctgggaat
     1681 ccaaagccaa agatctattg gtttaaagat gggaagcaga tttctccgaa gagcgatcac
     1741 tacaccattc agagagacct tgatgggacc tgctctctcc acaccacggc ctctacccta
     1801 gacgacgatg ggaactacac catcatggct gccaaccctc agggtcgcgt cagttgtaca
     1861 ggaaggctaa tggtacaggc tgtcaaccaa agaggccgca gtccccgctc tccctcaggc
     1921 catcctcatg ccagaaggcc tcgctctcga tcacgggaca gtggagatga aaacgagccc
     1981 attcaggagc gattcttcag acctcacttc ctgcaggctc ctggagacct gaccgttcag
     2041 gaaggcaagc tctgcaggat ggactgcaag gtcagtgggt taccaacccc agatctcagc
     2101 tggcaactag acggaaagcc catacgcccc gacagtgctc acaagatgct ggtccgtgag
     2161 aatggggtcc actccctcat tatagagcca gtcacgtccc gggacgccgg catctacaca
     2221 tgtattgcca ccaacagagc aggacagaac tcgtttaacc tggagcttgt ggttgctgct
     2281 aaggaagcac acaaggcccc tgtgtttatg gagaagctac agaacacggg ggttgctgat
     2341 ggatacccag tgcggctaga atgccgtgtc tccggagtgc cgccacctca gatattttgg
     2401 aagaaagaaa atgaatcgct cactcatagc actgagcgag taagcatgca ccaggataat
     2461 catggctaca tctgcctgct catccaggga gccacaaagg aagacgctgg gtggtacact
     2521 gtgtctgcca agaacgaagc aggcattgtg tcctgcactg cccggctgga tgtctacacc
     2581 cagtggcacc agcagccaca gaccaccaag ccaaaaaaag tacggccctc ggccagtcgc
     2641 tacgcagcac tttcggacca gggactagac atcaaagccg ctttccaacc tgaagccagc
     2701 ccatctcacc tgactctgaa cagcggcttg gtagaaagtg aagacctgtg agaattctgc
     2761 agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca
     2821 taccacatt
//
