LOCUS       sequence-85323-e        2507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, 
            Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, 
            Waterman CM
  TITLE     Vinculin-actin interaction couples actin retrograde flow to focal 
            adhesions, but is dispensable for focal adhesion growth.
  JOURNAL   J Cell Biol. 2013 Jul 8;202(1):163-77. doi: 10.1083/jcb.201303129.
  PUBMED    23836933
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-07-8- 8"; volume: "202"; 
            issue: "1"; pages: "163-77"
COMMENT     Sequence Label: mApple-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1692..1744
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1752..1761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1758..2468
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2171..2190
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag caccatggac gacctcgatg ccttactggc agacctggaa tccaccacct
       61 cgcatatctc taagcgaccg gtgtttctaa cagaggaaac gccttactcc tacccaactg
      121 gaaaccatac gtaccaggag attgctgtgc caccgccggt gcccccgccg ccttccagtg
      181 aggccctaaa tggcactgtg attgacccct tagaccagtg gcaacccagc gtgtccagat
      241 acggccacca gcaacctcag tcccagtctc cgatatacag ctctagtgcc aaaagctcca
      301 gtgcctctgt tcctagagac gggctcagct ccccttctcc ccgtgccagt gaggaggaac
      361 acgtgtacag cttcccaaac aagcagaagt ctgcagaacc atctcccaca atgaccagca
      421 cctccctggg cagcaacctc tcggaactgg acaggcttct cctggaactg aatgctgttc
      481 aacataatcc ccccagtggc ttctcagcag atgaagtcag cagaagccca tcactgccca
      541 atgtgaccgg acctcactat gtcatcccag agagcagcag ctctgcagga gggaaggctg
      601 caccccctac aaaagaaaaa ccaaagcgga atggtgggcg tgggattgaa gatgtgcgtc
      661 ccagtgtgga gagcctgctg gatgagctgg agagctctgt gccaagccca gtccctgcaa
      721 tcactgtgag ccaaggggag gtgagcagcc cccagcgtgt caacgccagt cagcagcaga
      781 cccgtatctc tgcttcttca gctacacgag aactggatga gctgatggcg tccctctctg
      841 actttaagtt catggcacag gggaaagccg gcggcagcag ctccccacca tccaccaccc
      901 caaagcctgg cagtcagctg gacaccatgc tgggaagtct gcagtctgac ctgaacaaac
      961 tgggggtagc gacggtcgcc aagggtgtgt gcggagcctg caagaagccg attgctgggc
     1021 aggtagttac agccatgggg aagacctggc accctgagca cttcgtctgc acccactgcc
     1081 aggaggagat tggatcacgg aacttctttg agcgggatgg gcagccctac tgtgagaagg
     1141 actatcacaa cctcttctct cctcgctgct actactgcaa tgggccaatc cttgataaag
     1201 tggtgacggc tttggacagg acgtggcacc ctgaacactt tttctgtgcc cagtgtggag
     1261 ttttctttgg acctgaagga tttcatgaga aggatggcaa agcctattgc cgcaaggact
     1321 actttgacat gtttgctccc aagtgtggag gctgtgcccg ggctatcctg gaaaattaca
     1381 tttctgcctt aaacaccctg tggcaccctg agtgctttgt ctgtcgggaa tgtttcacac
     1441 cgttcatcaa cggcagcttc ttcgagcacg acgggcagcc ctactgcgag gtgcattacc
     1501 acgagcgccg cggctcgctg tgctctggct gccagaagcc catcacggga cgctgcatca
     1561 ctgccatggg caagaaattt caccccgaac actttgtctg tgccttctgc ctcaagcagc
     1621 tcaacaaagg aaccttcaaa gaacagaacg acaagcccta ctgtcagaac tgctttctca
     1681 aactcttctg tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     1741 atccaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg
     1801 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     1861 agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg aaggtgacca
     1921 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     1981 aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc ttccccgagg
     2041 gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac gttaaccagg
     2101 actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc accaacttcc
     2161 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc gaggagcgga
     2221 tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg
     2281 gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2341 ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag gactacacca
     2401 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2461 acaagtaagc ggccgcgact ctagatcata atcagccata ccacatt
//
