LOCUS       sequence-85324-e        2510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2510)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Paxillin-KM-N-18
FEATURES             Location/Qualifiers
     source          1..2510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1755..1764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1761..2471
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2174..2193
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaacgtcag atccgctagc gccaccatga tggacgacct cgatgcctta ctggcagacc
       61 tggaatccac cacctcgcat atctctaagc gaccggtgtt tctaacagag gaaacgcctt
      121 actcctaccc aactggaaac catacgtacc aggagattgc tgtgccaccg ccggtgcccc
      181 cgccgccttc cagtgaggcc ctaaatggca ctgtgattga ccccttagac cagtggcaac
      241 ccagcgtgtc cagatacggc caccagcaac ctcagtccca gtctccgata tacagctcta
      301 gtgccaaaag ctccagtgcc tctgttccta gagacgggct cagctcccct tctccccgtg
      361 ccagtgagga ggaacacgtg tacagcttcc caaacaagca gaagtctgca gaaccatctc
      421 cctcactgac cagcacctcc ctgggcagca acctctcgga actggacagg cttctcctgg
      481 aactgaatgc tgttcaacat aatcccccca gtggcttctc agcagatgaa gtcagcagaa
      541 gcccatcact gcccaatgtg accggacctc actatgtcat cccagagagc agcagctctg
      601 caggagggaa ggctgcaccc cctacaaaag aaaaaccaaa gcggaatggt gggcgtggga
      661 ttgaagatgt gcgtcccagt gtggagagcc tgctggatga gctggagagc tctgtgccaa
      721 gcccagtccc tgcaatcact gtgagccaag gggaggtgag cagcccccag cgtgtcaacg
      781 ccagtcagca gcagacccgt atctctgctt cttcagctac acgagaactg gatgagctga
      841 tggcgtccct ctctgacttt aagttcatgg cacaggggaa agccggcggc agcagctccc
      901 caccatccac caccccaaag cctggcagtc agctggacac catgctggga agtctgcagt
      961 ctgacctgaa caaactgggg gtagcgacgg tcgccaaggg tgtgtgcgga gcctgcaaga
     1021 agccgattgc tgggcaggta gttacagcca tggggaagac ctggcaccct gagcacttcg
     1081 tctgcaccca ctgccaggag gagattggat cacggaactt ctttgagcgg gatgggcagc
     1141 cctactgtga gaaggactat cacaacctct tctctcctcg ctgctactac tgcaatgggc
     1201 caatccttga taaagtggtg acggctttgg acaggacgtg gcaccctgaa cactttttct
     1261 gtgcccagtg tggagttttc tttggacctg aaggatttca tgagaaggat ggcaaagcct
     1321 attgccgcaa ggactacttt gacatgtttg ctcccaagtg tggaggctgt gcccgggcta
     1381 tcctggaaaa ttacatttct gccttaaaca ccctgtggca ccctgagtgc tttgtctgtc
     1441 gggaatgttt cacaccgttc atcaacggca gcttcttcga gcacgacggg cagccctact
     1501 gcgaggtgca ttaccacgag cgccgcggct cgctgtgctc tggctgccag aagcccatca
     1561 cgggacgctg catcactgcc atgggcaaga aatttcaccc cgaacacttt gtctgtgcct
     1621 tctgcctcaa gcagctcaac aaaggaacct tcaaagaaca gaacgacaag ccctactgtc
     1681 agaactgctt tctcaaactc ttctgtagca gcggtggagc aagcgcagcc agtggtagcg
     1741 cggatccacc ggtcgccacc atggtgagca agggcgagga gaataacatg gccatcatca
     1801 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     1861 tcgagggcga gggcgagggc cgcccctacg aggcctttca gaccgctaag ctgaaggtga
     1921 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     1981 ccaaggtcta cattaagcac ccagccgaca tccccgacta cttcaagctg tccttccccg
     2041 agggcttcag gtgggagcgc gtgatgaact tcgaggacgg cggcattatt cacgttaacc
     2101 aggactcctc cctgcaggac ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact
     2161 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccgaggagc
     2221 ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa gaagaggctg aagctgaagg
     2281 acggcggcca ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     2341 tgcccggcgc ctacatcgtc gacatcaagt tggacatcgt gtcccacaac gaggactaca
     2401 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     2461 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
//
