LOCUS       sequence-85325-e        1677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1677)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-PCNA-19-NLS-4
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..60
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..786
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     492..511
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagccac catggcttcg tggggctccc cgaagaagaa gcgcaaagta
       61 ctggtaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
      121 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      181 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
      241 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      301 aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
      361 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag
      421 gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg caccaacttc
      481 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc cgaggagcgg
      541 atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa gctgaaggac
      601 ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      661 cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc
      721 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      781 tacaagtccg gagaagggca agggcaaggg caagggccgg gccgcggcta cgcgtatcga
      841 tccatgttcg aggcgcgcct ggtccagggc tccatcctca agaaggtgtt ggaggcactc
      901 aaggacctca tcaacgaggc ctgctgggat attagctcca gcggtgtaaa cctgcagagc
      961 atggactcgt cccacgtctc tttggtgcag ctcaccctgc ggtctgaggg cttcgacacc
     1021 taccgctgcg accgcaacct ggccatgggc gtgaacctca ccagtatgtc caaaatacta
     1081 aaatgcgccg gcaatgaaga tatcattaca ctaagggccg aagataacgc ggataccttg
     1141 gcgctagtat ttgaagcacc aaaccaggag aaagtttcag actatgaaat gaagttgatg
     1201 gatttagatg ttgaacaact tggaattcca gaacaggagt acagctgtgt agtaaagatg
     1261 ccttctggtg aatttgcacg tatatgccga gatctcagcc atattggaga tgctgttgta
     1321 atttcctgtg caaaagacgg agtgaaattt tctgcaagtg gagaacttgg aaatggaaac
     1381 attaaattgt cacagacaag taatgtcgat aaagaggagg aagctgttac catagagatg
     1441 aatgaaccag ttcaactaac ttttgcactg aggtacctga acttctttac aaaagccact
     1501 ccactctctt caacggtgac actcagtatg tctgcagatg taccccttgt tgtagagtat
     1561 aaaattgcgg atatgggaca cttaaaatac tacttggctc ccaagatcga ggatgaagaa
     1621 ggatcttagg gatccaccgg atctagataa ctgatcataa tcagccatac cacattt
//
