LOCUS       sequence-85326-e        1958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1958)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1210..1219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1216..1926
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1629..1648
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcaccg ccaccatgag gaagatgctc gccgccgtct cccgcgtgct gtctggcgct
       61 tctcagaagc cggcaagcag agtgctggta gcatcccgta attttgcaaa tgatgctaca
      121 tttgaaatta agaaatgtga ccttcaccgg ctggaagaag gccctcctgt cacaacagtg
      181 ctcaccaggg aggatgggct caaatactac aggatgatgc agactgtacg ccgaatggag
      241 ttgaaagcag atcagctgta taaacagaaa attattcgtg gtttctgtca cttgtgtgat
      301 ggtcaggaag cttgctgtgt gggcctggag gccggcatca accccacaga ccatctcatc
      361 acagcctacc gggctcacgg ctttactttc acccggggcc tttccgtccg agaaattctc
      421 gcagagctta caggacgaaa aggaggttgt gctaaaggga aaggaggatc gatgcacatg
      481 tatgccaaga acttctacgg gggcaatggc atcgtgggag cgcaggtgcc cctgggcgct
      541 gggattgctc tagcctgtaa gtataatgga aaagatgagg tctgcctgac tttatatggc
      601 gatggtgctg ctaaccaggg ccagatattc gaagcttaca acatggcagc tttgtggaaa
      661 ttaccttgta ttttcatctg tgagaataat cgctatggaa tgggaacgtc tgttgagaga
      721 gcggcagcca gcactgatta ctacaagaga ggcgatttca ttcctgggct gagagtggat
      781 ggaatggata tcctgtgcgt ccgagaggca acaaggtttg ctgctgccta ttgtagatct
      841 gggaaggggc ccatcctgat ggagctgcag acttaccgtt accacggaca cagtatgagt
      901 gaccctggag tcagttaccg tacacgagaa gaaattcagg aagtaagaag taagagtgac
      961 cctattatgc ttctcaagga caggatggtg aacagcaatc ttgccagtgt ggaagaacta
     1021 aaggaaattg atgtggaagt gaggaaggag attgaggatg ctgcccagtt tgccacggcc
     1081 gatcctgagc cacctttgga agagctgggc taccacatct actccagcga cccacctttt
     1141 gaagttcgtg gtgccaatca gtggatcaag tttaagtcag tcagtagctc cggaggggat
     1201 ccaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat catcaaggag
     1261 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     1321 ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa ggtgaccaag
     1381 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1441 gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt ccccgagggc
     1501 ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt taaccaggac
     1561 tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1621 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga ggagcggatg
     1681 taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct gaaggacggc
     1741 ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1801 ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc
     1861 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1921 aagtaagcgg ccgcgactct agatcataat cagccata
//
