LOCUS       sequence-85327-e        1398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             654..1364
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1067..1086
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcgatccgc tagcgctacc ggactcagat ctgccaccat ggcgccggcc gcgtccaggc
       61 tgcgggccga agccgggctc ggggcgctgc cgcggcgggc gctcgcccag tacttgctct
      121 tcctgcggct ctacccggtg ctcaccaagg cggccaccag tggcattttg tcagcacttg
      181 ggaacttcct ggcccagatg attgagaaga agcggaaaaa agaaaactct agaagtctgg
      241 atgtcggtgg gcctctgaga tatgccgttt acgggttctt cttcacaggg ccgctgagtc
      301 acttcttcta cttcttcatg gaacattgga tccctcctga ggtccccctg gcagggctca
      361 ggaggcttct cctggaccgc ctcgtctttg caccggcctt cctcatgttg ttcttcctca
      421 tcatgaactt tctggagggg aaagacgcct cagccttcgc cgccaagatg agggggggct
      481 tctggccggc gctgaggatg aactggcggg tgtggacgcc actacagttc atcaacatca
      541 actacgtccc tctgaagttc cgggtgctct tcgccaacct ggcagctctg ttctggtatg
      601 cctacctggc ctccttgggg aagagcggct ccggtagcgg accggtcgcc accatggtga
      661 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      721 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      781 acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      841 gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg
      901 acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga
      961 acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt
     1021 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1081 agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga
     1141 agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca
     1201 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca
     1261 agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1321 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     1381 atcataatca gccatacc
//
