LOCUS       sequence-85328-e        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mApple-Profilin-10
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..736
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     442..461
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc
       61 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      121 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
      181 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      241 gtacggctcc aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
      301 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
      361 cgttaaccag gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg
      421 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      481 cgaggagcgg atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa
      541 gctgaaggac ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc
      601 cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga
      661 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttccatgg ccgggtggaa
      781 cgcctacatc gacagcctta tggcggacgg gacctgtcag gacgcggcca tcgtaggcta
      841 caaggactcg ccctccgtct gggccgccgt ccccgggaag accttcgtta gcattacgcc
      901 agctgaggtt ggtgtcctgg taggcaaaga ccggtcaagt tttttcgtca atgggctgac
      961 acttgggggc cagaaatgtt ctgtgatccg ggactcactg ctgcaagacg gggaatttac
     1021 aatggatctt cgtaccaaga gcaccggagg agcccccacc ttcaatgtca ctgtcaccat
     1081 gactgccaag acgctagtcc tgctgatggg caaagaaggt gtccacggtg gtttgatcaa
     1141 caagaaatgt tatgaaatgg cctctcacct gcggcgttcc cagtactaag gatccaccgg
     1201 atctagataa ctgatcataa tcagccatac cacatt
//
