LOCUS       sequence-85329-e        2986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2986)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..2986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2243..2252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2249..2959
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2662..2681
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgaggc caccatggac tgtctctgta tagtgacaac
       61 caagaaatac cgctaccaag atgaagacac gccccctctg gaacacagcc cggcccacct
      121 ccccaaccag gccaattctc cccctgtgat tgtcaacacg gacaccctag aagccccagg
      181 atatgagttg caggtgaatg gaacagaggg ggagatggag tatgaggaga tcacattgga
      241 aaggggtaac tcaggtctgg gcttcagcat cgcaggtggc actgacaacc cgcacatcgg
      301 tgacgacccg tccattttta tcaccaagat cattcctggt ggggctgcag cccaggatgg
      361 ccgcctcagg gtcaatgaca gcatcctgtt tgtaaatgaa gtggatgttc gggaggtgac
      421 ccattcagct gcggtggagg ccctcaaaga ggcaggttcc atcgttcgcc tctatgtcat
      481 gcgccggaaa cccccagccg aaaaggtcat ggagatcaaa ctcatcaaag ggcctaaagg
      541 acttggcttc agcattgcgg ggggcgttgg gaaccagcac atccctggag ataacagcat
      601 ctatgtaacg aagatcatcg aaggaggtgc tgcccacaag gatggcaggt tgcagattgg
      661 agacaagatc ctggcggtca acagtgtggg gctggaggac gtcatgcacg aggatgccgt
      721 ggcagccctg aagaacacat atgacgttgt gtacctaaag gtggccaagc ccagcaatgc
      781 ctacctgagt gacagctatg ctcccccaga catcacaacc tcgtattctc agcacctgga
      841 caatgagatc agtcatagca gctacttggg cactgactac cccacagcca tgacccccac
      901 ttcccctcgg cgctactccc ctgtggccaa ggacctgctg ggggaggaag acattccccg
      961 ggaaccaagg cggatcgtga tccatcgggg ctccaccggc ctgggcttca acatcgtggg
     1021 cggcgaggat ggtgaaggca tcttcatctc cttcatcctt gctgggggtc cagccgacct
     1081 cagtggggag ctacggaagg gggaccagat cctgtcggtc aatggtgttg acctccgcaa
     1141 tgccagtcac gaacaggctg ccattgccct gaagaatgcg ggtcagacgg tcacgatcat
     1201 cgctcagtat aaaccagaag agtatagtcg attcgaggcc aagatccatg atcttcggga
     1261 acagctcatg aatagtagcc taggctcagg gactgcatcc ttgcgaagca accccaagag
     1321 gggcttctac attagggccc tgtttgatta cgacaagacc aaggactgcg gtttcttgag
     1381 ccaggccctg agcttccgct tcggggatgt gcttcatgtc attgacgctg gtgacgaaga
     1441 gtggtggcaa gcacggcggg tccactccga cagtgagacc gacgacattg gcttcattcc
     1501 cagcaaacgg cgggtcgagc gacgagagtg gtcaaggtta aaggccaagg actggggctc
     1561 cagctctgga tcacagggtc gagaagactc ggttctgagc tatgagacgg tgacccagat
     1621 ggaagtgcac tatgctcgtc ccatcatcat ccttggaccc accaaagacc gtgccaacga
     1681 tgatcttctc tccgagttcc ccgacaagtt tggatcctgt gtccctcata cgacacgtcc
     1741 taagcgggaa tatgagatag acggccggga ttaccacttt gtctcctccc gggagaaaat
     1801 ggagaaggac atccaggcac acaagttcat tgaggctggc cagtacaaca gccacctcta
     1861 tgggaccagc gtccagtctg tgcgagaggt agcagagcag gggaagcact gcatcctcga
     1921 tgtctcggcc aatgccgtgc ggcggctgca ggcggcccac ctgcacccca tcgccatctt
     1981 catccgtccc cgctccctgg agaatgtgct agagatcaat aagcggatca cagaggagca
     2041 agcccggaaa gccttcgaca gagccacgaa gctggagcag gagttcacag agtgcttctc
     2101 agccatcgta gagggcgaca gctttgaaga gatctatcac aaagtgaaac gtgtcattga
     2161 agacctctca ggcccctaca tctgggtccc agcccgagag agactcggca gcgcatccgg
     2221 cggaagcgga agcggaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc
     2281 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     2341 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
     2401 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     2461 gtacggctcc aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
     2521 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
     2581 cgttaaccag gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg
     2641 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     2701 cgaggagcgg atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa
     2761 gctgaaggac ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc
     2821 cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga
     2881 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     2941 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcag
//
