LOCUS       sequence-85330-e        1460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1460)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Rab11a-7
FEATURES             Location/Qualifiers
     source          1..1460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgggcac ccgcgacgac
      781 gagtacgact acctctttaa agttgtcctt attggagatt ctggtgttgg aaagagtaat
      841 ctcctgtctc gatttactcg aaatgagttt aatctggaaa gcaagagcac cattggagta
      901 gagtttgcaa caagaagcat ccaggttgat ggaaaaacaa taaaggcaca gatatgggac
      961 acagcagggc aagagcgata tcgagctata acatcagcat attatcgtgg agctgtaggt
     1021 gccttattgg tttatgacat tgctaaacat ctcacatatg aaaatgtaga gcgatggctg
     1081 aaagaactga gagatcatgc tgatagtaac attgttatca tgcttgtggg caataagagt
     1141 gatctacgtc atctcagggc agttcctaca gatgaagcaa gagcttttgc agaaaagaat
     1201 ggtttgtcat tcattgaaac ttcggcccta gactctacaa atgtagaagc tgcttttcag
     1261 acaattttaa cagagattta ccgcattgtt tctcagaagc aaatgtcaga cagacgcgaa
     1321 aatgacatgt ctccaagcaa caatgtggtt cctattcatg ttccaccaac cactgaaaac
     1381 aagccaaagg tgcagtgctg tcagaacatc taaggatcca ccggatctag ataactgatc
     1441 ataatcagcc ataccacatg
//
