LOCUS       sequence-85331-e        1466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1466)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gccatgtcgc agacggccat
      781 gtccgaaacc tacgattttt tgtttaagtt cttggttatt ggaaatgcag gaactggcaa
      841 atcttgctta cttcatcagt ttattgaaaa aaaattcaaa gatgactcaa atcatacaat
      901 aggagtggaa tttggttcaa agataataaa tgttggtggt aaatatgtaa agttacaaat
      961 atgggataca gcaggacaag aacgattcag gtccgtgacg agaagttatt accgaggcgc
     1021 ggccggggct ctcctcgtct atgatatcac cagccgagaa acctacaatg cgcttactaa
     1081 ttggttaaca gatgcccgaa tgctggcgag ccagaacatt gtgatcatcc tttgtggaaa
     1141 caagaaggac ctggatgcag atcgtgaagt taccttctta gaagcctcca gatttgctca
     1201 agaaaatgag ctgatgtttt tggaaacaag tgcgctcaca ggggagaatg tagaagaggc
     1261 ttttgtacag tgtgcaagaa aaatacttaa caaaatcgaa tcaggtgagc tggacccaga
     1321 aagaatgggc tcaggtattc agtacggaga tgctgccttg agacagctga ggtcaccgcg
     1381 gcgcgcacag gccccgaacg ctcaggagtg tggttgttag ggatccaccg gatctagata
     1441 actgatcata atcagccata ccacat
//
