LOCUS       sequence-85332-e        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ccatggctag tcgaggcgca
      781 acaagaccca acgggccaaa tactggaaat aaaatatgcc agttcaaact agtacttctg
      841 ggagagtctg ctgttggcaa atcaagccta gtgcttcgtt ttgtgaaggg ccaatttcat
      901 gaatttcaag agagtaccat aggggctgct tttctaaccc aaactgtgtg tcttgatgat
      961 acaacagtaa agtttgaaat atgggataca gctggtcaag aacgatacca tagcttagca
     1021 ccaatgtact acagaggagc acaagcagcc atagttgtat atgatatcac aaatgaggag
     1081 tcctttgcca gagccaaaaa ctgggttaaa gaacttcaga ggcaagccag tcctaacatt
     1141 gtaatagctt tatcaggaaa caaggctgat cttgcaaata aaagagctgt cgatttccag
     1201 gaagcacagt cctatgcaga tgacaacagt ttattattca tggagacatc agctaaaaca
     1261 tcgatgaatg taaatgaaat attcatggca atagctaaaa agttgccaaa gaacgaacca
     1321 cagaatccag gagcaaattc tgccagagga agaggagtag accttactga acccacgcag
     1381 ccaaccagga atcagtgttg tagtaactaa ggatccaccg gatctagata actgatcata
     1441 atcagccata ccacatt
//
