LOCUS       sequence-85333-e        1420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1420)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc
      301 ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac
      361 gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag
      541 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct cgagccatga cctctaggaa gaaagtgttg
      781 ctgaaggtta tcatcctggg agattctgga gtcgggaaga catcactcat gaaccagtat
      841 gtgaataaga aattcagcaa tcagtacaaa gccacaatag gagctgactt tctgaccaag
      901 gaggtgatgg tggatgacag gctagtcaca atgcagatat gggacacagc aggacaggaa
      961 cggttccagt ctctcggtgt ggccttctac agaggtgcag actgctgcgt tctggtattt
     1021 gatgtgactg cccccaacac attcaaaacc ctagatagct ggagagatga gtttctcatc
     1081 caggccagtc cccgagatcc tgaaaacttc ccatttgttg tgttgggaaa caagattgac
     1141 ctcgaaaaca gacaagtggc cacaaagcgg gcacaggcct ggtgctacag caaaaacaac
     1201 attccctact ttgagaccag tgccaaggag gccatcaacg tggagcaggc gttccagacg
     1261 attgcacgga atgcacttaa gcaggaaacg gaggtggagc tgtacaacga atttcctgaa
     1321 cctatcaaac tggacaagaa tgaccgggcc aaggcctcgg cagaaagctg cagttgctga
     1381 ggatccaccg gatctagata actgatcata atcagccata
//
