LOCUS       sequence-85334-e        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Sec61-C-18
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             795..1082
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagaataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
      181 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc
      301 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
      361 ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc
      541 tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctggctc cagcgcaggc agcgcatccg
      781 gcggaagcgg aagccctggt ccgaccccca gtggcactaa cgtgggatcc tcagggcgct
      841 ctcccagcaa agcagtggcc gcccgggcgg cgggatccac tgtccggcag aggaaaaatg
      901 ccagctgtgg gacaaggagt gcaggccgca caacctcggc aggcaccggg gggatgtggc
      961 gattctacac agaagattca cctgggctca aagttggccc tgttccagta ttggttatga
     1021 gtcttctgtt catcgcttct gtatttatgt tgcacatttg gggcaagtac actcgttcgt
     1081 aggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
     1141 ataatcagcc ata
//
