LOCUS       sequence-85335-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..323
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     regulatory      372..381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             378..1088
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     791..810
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcgtgctag tgacgtcaga tccgctagcg ccaccatgcc tggtccgacc cccagtggca
       61 ctaacgtggg atcctcaggg cgctctccca gcaaagcagt ggccgcccgg gcggcgggat
      121 ccactgtccg gcagaggaaa aatgccagct gtgggacaag gagtgcaggc cgcacaacct
      181 cggcaggcac cggggggatg tggcgattct acacagaaga ttcacctggg ctcaaagttg
      241 gccctgttcc agtattggtt atgagtcttc tgttcatcgc ttctgtattt atgttgcaca
      301 tttggggcaa gtacactcgt tcgggacgaa ttctgcagtc gacggtaccg cgggcccggg
      361 atccaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg
      421 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      481 agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg aaggtgacca
      541 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      601 aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc ttccccgagg
      661 gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac gttaaccagg
      721 actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc accaacttcc
      781 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc gaggagcgga
      841 tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg
      901 gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      961 ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag gactacacca
     1021 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     1081 acaagtaagc ggccgcgact ctagatcata atcagccata ccacattgta gagtcc
//
