LOCUS       sequence-85337-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1381..2091
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1794..1813
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatggc cactgttacc
       61 tatccttcat ctgagcctac gaccttggac cctgggaacg catcctcgac ctggcccctg
      121 gataccaccc tggggaacac atccgctggc gctagcctga caggcctggc tgtcagtggc
      181 atcttgatct ctctggtgta cctggtggtg tgcgtggtgg gtttgctggg caactcgctg
      241 gtgatctacg tggtcctgcg gcacacgtcc agcccatcag tgaccagtgt ctatatcctc
      301 aacctggctc tggctgatga gctcttcatg ctagggctac ccttcctggc tgctcagaac
      361 gccctgtcct actggccctt cggatctctc atgtgccgtc tggtcatggc cgtggatggc
      421 atcaaccagt tcaccagcat cttctgcctc accgtcatga gtgtggaccg ctatctggct
      481 gtggtgcacc ccacacgctc agcccgctgg cgcacggcac cagtggctcg cacggtcagt
      541 gcagctgtct gggtggcctc ggctgtggtg gtgctgcctg tggttgtgtt ctcaggagtg
      601 ccccggggca tgagcacgtg ccacatgcag tggccagagc cagcggctgc ctggcgaaca
      661 gccttcatca tctacacggc cgcactgggc ttctttgggc ccctgctggt catctgcttg
      721 tgctacttgc tcattgtggt aaaggtgcgg tcgaccaccc ggcgggtgcg ggcgccctcg
      781 tgtcagtggg tacaggcacc cgcatgccag cggcgacgcc gctctgagcg cagggtcaca
      841 cgcatggtgg tggccgtggt ggcactcttc gtcctctgct ggatgccttt ctatctgctc
      901 aacatcgtca atgtggtgtg cccgctgccg gaggagcccg ccttcttcgg cctctacttc
      961 ctggtggtgg cgctgcccta cgccaacagc tgcgcaaacc ccatcctcta cggcttcctc
     1021 tcctaccgct tcaagcaggg ctttcgcagg atcctgctaa gaccatcacg tcgcattcgg
     1081 agccaggagc cagggtcggg acctccagag aagactgaag aggaggagga tgaagaagaa
     1141 gaagagagaa gggaagagga ggagcggagg atgcagagag ggcaggagat gaacgggagg
     1201 ctcagtcaga tcgcacaggc tggcactagt ggacaacagc cacggccctg cacagggact
     1261 gctaaggagc agcagcttct gccccaggag gccacagctg gggacaaggc cagcacactg
     1321 agccatctgg gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     1381 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
     1441 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1501 cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
     1561 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac
     1621 ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc
     1681 gtgatgaact tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac
     1741 ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1801 atgcagaaga agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc
     1861 gccctgaaga gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc
     1921 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
     1981 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa
     2041 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg
     2101 actctagatc ataatcagcc ataccacatt
//
