LOCUS       sequence-85339-e        8466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8466)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Talin-N-10
FEATURES             Location/Qualifiers
     source          1..8466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7711..7720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7717..8427
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     8130..8149
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctagcc
       61 accatggttg cgctttcgct gaagattagc attgggaatg tggtgaagac gatgcaattt
      121 gagccatcta ccatggtgta cgacgcctgc cgcatgattc gtgagcggat cccagaggcc
      181 ctggctggcc ctcccaacga ctttgggctc tttctgtcag atgatgaccc caaaaaaggc
      241 atctggctag aggctgggaa agctttggac tactacatgc tccgaaatgg ggacaccatg
      301 gagtacagaa aaaagcagag acccctgaag atacggatgt tagacggaac ggtgaagact
      361 atcatggtgg atgactccaa gaccgtcaca gacatgctca tgaccatttg tgcccgaatt
      421 ggtatcacca accatgatga gtattcactg gttcgagagc tgatggaaga aaagaaagat
      481 gaggggacag ggaccctgag aaaagacaag accctactgc gagatgaaaa gaagatggag
      541 aagctaaagc agaagttgca cacagacgat gagttgaact ggctggacca tgggcggaca
      601 ctgagggaac agggagtgga agagcatgag acgttgctgc tgcggagaaa gttcttctac
      661 tcagaccaga atgtggattc ccgagaccct gtacagctga accttctcta tgtgcaggca
      721 cgagatgaca tcctgaatgg ctcccatcct gtctcctttg acaaggcctg tgaatttgca
      781 ggcttccagt gccagatcca gtttggacct cacaatgaac agaagcacaa ggctggcttc
      841 cttgacctga aggacttcct gcccaaggag tatgtgaagc agaagggaga gcgtaagatc
      901 tttcaggcac acaagaattg tgggcagatg agtgaaattg aggccaaggt acgctacgtg
      961 aagctggccc gttccctcaa gacttacggt gtctccttct tcctagtcaa ggaaaagatg
     1021 aaggggaaga ataaactggt gcccaggctg ttgggcatca ctaaggagtg tgtgatgcgt
     1081 gtggatgaga agaccaagga ggtgattcag gaatggagcc tcaccaacat caaacgctgg
     1141 gctgcctctc ccaagagctt tactctggac tttggagact atcaggatgg ctactactca
     1201 gtacagacga cagaaggcga gcagatcgca cagctcattg ctggctacat agatatcatc
     1261 cttaagaaga aaaaaagcaa ggaccatttt gggctggagg gagatgaaga gtctactatg
     1321 ttggaggact cagtgtctcc caaaaagtca acagtccttc agcagcagta caaccgagtg
     1381 gggaaagtgg agcacggctc tgtggctctg ccagccatca tgcgctctgg agcctctggt
     1441 cctgagaatt tccaggtggg gagcatgcca cctgcccagc agcagatcac cagtggccag
     1501 atgcaccgag gacacatgcc acctctgact tcagcccagc aggcgctcac tggaaccatt
     1561 aactccagca tgcaggctgt gcaggctgcc caggccactc tggatgactt tgagactcta
     1621 ccccctcttg gccaggatgc tgcctccaaa gcttggcgta agaacaagat ggatgaatca
     1681 aagcatgaga tccactccca ggtagatgcc atcacagctg gcactgcctc tgtggtaaac
     1741 ctgacagcag gagatcctgc agagacagac tatacagcag tgggctgtgc agtcaccacc
     1801 atctcttcca acctgacgga gatgtcccgt ggggtgaagc tattggctgc cctgctggag
     1861 gatgaaggcg gcaatggccg gccccttctg caagcagcaa agggccttgc aggggctgtg
     1921 tcggaactgc ttcgcagtgc tcagcccgcc agcgctgagc ctcgtcagaa cctgctgcag
     1981 gcagccggga acgtgggcca ggccagtggg gagctgttgc agcaaattgg ggaaagtgac
     2041 actgaccccc acttccagga tgttctaatg cagctagcaa acgcagtggc aagtgctgca
     2101 gccgccctgg tcctcaaggc caagagtgtg gcccagcgca cagaggattc cgggcttcag
     2161 acccaagtga ttgctgcagc tacgcaatgt gctctgtcca cttcccagct ggtggcctgt
     2221 accaaggtgg tagcacctac aatcagctca cctgtgtgcc aagaacagct agtcgaggct
     2281 gggcgactgg tggccaaagc tgtggagggc tgtgtgtctg cctcgcaagc agctacagaa
     2341 gatggacagc tgcttcgagg ggtaggagca gcagccacgg ctgtcaccca ggccctcaat
     2401 gagctgctgc agcacgtgaa ggcccacgcc accggagctg ggcctgccgg tcggtatgac
     2461 caagccactg ataccatcct caccgtcact gaaaacatct tcagctccat gggtgatgct
     2521 ggggagatgg tgcgccaggc ccgcatcctg gcccaagcca cctcagacct ggtcaatgct
     2581 atcaaggccg atgctgaggg ggagagtgat ctggagaact ctagaaagct cctgagtgct
     2641 gccaagatcc tcgctgatgc caccgccaag atggtggagg cggccaaggg agcagccgcc
     2701 caccctgaca gtgaggaaca gcagcagcga ctgcgtgaag cagctgaggg gcttcgcatg
     2761 gccaccaatg cagctgcgca gaacgccatc aagaagaagt tggtgcagcg cctggagcat
     2821 gcagccaagc aagctgcagc ctctgcaaca cagaccattg ctgcagccca acatgcagcc
     2881 tctgccccca aggcctctgc gggcccccag cccctactgg tgcagagctg taaggccgtg
     2941 gcagagcaga ttccgttgct ggtgcagggt gtccgaggaa gtcaagctca gcctgacagc
     3001 cctagtgctc agctcgccct cattgctgcc agccagagct tcctgcagcc aggtgggaag
     3061 atggtggcag cggcaaaagc ctcagtgcca acaattcagg accaggcttc agcgatgcag
     3121 ctgagtcagt gcgcaaagaa cctaggcact gccctggccg agctgcgcac tgctgctcag
     3181 aaggctcagg aggcatgtgg acctttggag atggattctg cactgagtgt tgtacaaaat
     3241 ctagagaaag acctacagga aataaaagca gcagctcgag atggcaagct taagccctta
     3301 cctggggaaa caatggagaa gtgtacccaa gatcttggca acagcaccaa agcggtgagc
     3361 tctgccatcg ccaagctgct gggggagatt gcccagggca acgagaacta tgcaggtatt
     3421 gcagctcggg atgtagctgg tggactaagg tcactagccc aggctgcgcg gggtgtggct
     3481 gcgctgacat cagatcctgc agtgcaggcc attgtgcttg acacagccag cgacgttctg
     3541 gacaaggcca gcagcctcat tgaagaggca aaaaaagcat ctggacaccc aggggaccca
     3601 gaaagccagc aaaggcttgc tcaggtagct aaagcagtga cccaagccct gaaccgctgt
     3661 gtcagctgcc tgcctggcca aagagacgtg gataatgccc tacgagccgt tggagatgcc
     3721 agcaagcgac tcctgagtga cttgcttcct ccaagcacgg ggacatttca agaagcacag
     3781 agccgattga atgaagctgc agctgggtta aatcaggccg ccacagaact ggtgcaagcc
     3841 tcccgaggaa ctcctcagga cctggctcgg gcctccggcc gatttggaca ggacttcagc
     3901 accttcctgg aagctggtgt ggagatggcc ggacaggccc cgagccagga ggaccgagcc
     3961 caggtggtgt ccaacctgaa gggcatatcc atgtcttcaa gcaaacttct ccttgccgct
     4021 aaggccttgt ccacagaccc tgcttctccc aacctcaaga gtcagctggc tgcggctgcc
     4081 cgggcagtga cggacagcat caaccagctc atcaccatgt gcacccagca ggcacctggc
     4141 cagaaggagt gtgacaatgc acttcggcag ctagagacag tccgagaact cctggagaat
     4201 ccagtccagc ccatcaacga catgtcctac ttcggttgct tggacagtgt catggagaac
     4261 tccaaggtcc taggtgaggc catgactggc atctcccaaa atgccaagaa tggaaatctg
     4321 ccggagtttg gagatgccat tgccacagcc tccaaggctc tctgtggctt cacggaggca
     4381 gccgcacagg cagcatatct agttggtgtc tctgacccca acagccaagc tggacagcaa
     4441 ggactggtgg aacccacaca gtttgcccgt gcaaaccagg caattcagat ggcctgtcag
     4501 agtctggggg agcctggctg tacccaggcc caggtgttat ctgcagccac tattgtagcc
     4561 aaacacacat ctgcattgtg taacagctgt cgcctggctt ccgctaggac tgccaatccc
     4621 actgccaagc gccagtttgt acagtcagcc aaggaggtgg ccaacagtac agccaatctt
     4681 gtcaagacca tcaaggcact agatggggac ttcacagaag agaaccgtgc ccagtgccga
     4741 gcagccacag cccctctgct ggaagctgtg gataacctga gtgcctttgc ctccaaccct
     4801 gagttctcca gcgtccctgc ccagatcagc cctgagggca gagcagccat ggagcccatt
     4861 gtaatctctg ctaagacaat gttggagagt gctggagggc tcatccagac agctcgggcg
     4921 ttagcagtca atccccgaga ccccccacgt tggtctgtgc tagctggcca ctctcggact
     4981 gtctcagatt ccatcaagaa acttattaca agcatgaggg acaaagcccc agggcagctg
     5041 gagtgtgaga cagccattgc ggctctgaac agctgcttgc gggacctaga tcaggcttcg
     5101 cttgccgctg tcagccagca gcttgctccc cgtgaaggaa tctctcaaga ggctttgcac
     5161 acccagatgc tcactgcagt gcaagaaatt tctcatctca ttgagccgct ggccagcgct
     5221 gctcgggctg aagcctccca gctgggacac aaggtgtccc aaatggccca gtactttgaa
     5281 ccactcaccc tggctgcagt gggtgctgcg tctaagaccc tgagccaccc gcagcagatg
     5341 gcacttctgg accagactaa aacgttggca gagtctgcct tgcagttgct atatactgcc
     5401 aaggaggctg gtggtaaccc caagcaagca gcacacaccc aggaagccct ggaggaggct
     5461 gtgcagatga tgacagaggc cgtagaagac ctgacgacga ccctcaacga agcagccagt
     5521 gctgcaggag tcgttggcgg catggtggac tctatcactc aggccatcaa ccagctagat
     5581 gaaggaccta tgggtgaccc agaaggctca ttcgtagatt accagacaac catggtgagg
     5641 acagccaagg ccattgctgt cactgttcag gagatggtaa ccaagtcaaa caccagccct
     5701 gaagagctag gccctcttgc caaccagctg accagtgact atggccgact ggcctcacaa
     5761 gccaagcctg cagctgtggc tgctgaaaat gaagagatag gcgctcacat caaacaccga
     5821 gtacaggagc tgggccatgg ctgctctgct ctggtcacca aggcaggtgc cttgcagtgt
     5881 agccccagtg atgtctacac caagaaggag ctcatagagt gtgcccgcag agtgtcagag
     5941 aaggtctccc atgttctggc tgcactccag gctgggaatc gtggtaccca ggcctgcatt
     6001 acagcagcca gtgctgtgtc tggtatcatt gctgacctcg acaccaccat catgttcgct
     6061 actgctggca cacttaaccg tgagggtgct gaaacttttg ctgaccaccg ggagggtatc
     6121 ttaaagacag caaaggttct tgtggaggac accaaggtcc tagtgcagaa tgcagctggg
     6181 agccaggaga agttggcaca agccgcccag tcctccgtgg ccaccattac ccgcctcgct
     6241 gatgtggtca agctcggtgc agccagccta ggagccgaag accctgaaac tcaggtggtg
     6301 ctgatcaatg cagtaaagga cgtagccaag gccctgggtg acctcatcag cgctacgaag
     6361 gctgcagcgg gcaaagttgg ggatgaccct gcagtgtggc agctcaagaa ctctgccaag
     6421 gtgatggtga ccaatgtgac atcattgctc aagacagtga aggctgtgga agatgaggcc
     6481 accaaaggca cacgggccct agaggcaacc acagagcaca tacgtcagga actggcggtc
     6541 ttctgttccc cagagccacc tgccaagacc tctacccctg aagacttcat ccgaatgacc
     6601 aagggtatta ctatggcaac agccaaagcc gttgctgctg gcaattcctg tcgacaggaa
     6661 gatgtcattg ccacagccaa tctgagccga cgggctattg cggacatgct tcgggcttgc
     6721 aaggaagcag ctttccaccc agaagtggca cctgatgtac ggctccgagc cctgcactat
     6781 ggccgggagt gtgccaatgg ttacctggaa ctgctggacc acgtgctgct gacccttcag
     6841 aagccaaacc cagacctgaa gcagcagcta actggacact caaagcgggt tgctggctct
     6901 gtgactgagc tcatccaggc tgctgaagcc atgaaaggaa cagagtgggt ggacccagag
     6961 gaccctactg tcattgctga gaatgagctc ttgggagccg cagccgccat cgaggctgca
     7021 gccaagaagc tggagcagct gaagcccagg gccaaaccca aggaggcgga tgagtccttg
     7081 aactttgagg aacaaatcct agaagctgcc aagtccatcg ctgcagccac cagtgcactg
     7141 gtaaaggctg cgtcagcagc ccagagggag ctggtggctc aaggaaaggt gggcgccatt
     7201 ccagccaatg cactggatga tgggcagtgg tcgcagggcc tcatttccgc tgcccgtatg
     7261 gtggctgcgg ccaccaacaa tctgtgtgaa gcagctaacg cagctgtcca gggccatgct
     7321 agccaggaga aactcatttc ctcagccaag caagtagctg cctccacagc tcagctcctg
     7381 gtagcttgca aggtcaaagc agatcaggac tctgaggcaa tgaaacggct ccaggctgct
     7441 ggcaatgcag tgaagagggc ctcagataac ctggtaaagg cggcccagaa agctgcagcc
     7501 ttcgaagacc aggagaatga gacggtggtg gtgaaggaga aaatggttgg gggcattgcc
     7561 cagattatcg cagcacagga agagatgctt cggaaggaac gagagctgga agaggctcgg
     7621 aaaaagctcg cccagatccg gcagcagcag tacaagttct tgccttcaga gcttcgagac
     7681 gagcgcggct ccgcgtcccg atcaccggtc gccaccatgg tgagcaaggg cgaggagaat
     7741 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     7801 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc
     7861 gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     7921 cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc
     7981 aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc
     8041 attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag
     8101 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     8161 gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag
     8221 aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc
     8281 aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc
     8341 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     8401 ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     8461 cacatt
//
