LOCUS       sequence-8534-ex        5688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCG142
FEATURES             Location/Qualifiers
     source          1..5688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3733..3857
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3929..3948)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3930..3948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3953..3969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3953..3969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4071..4090)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4082..4891
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4149..4168)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5038..5626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5527..5546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgta ggatctggaa gtaaaatatt gtaatatttt
      721 ccctaaattt cacttaaaaa taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa
      781 attgacgatt tttgttcaaa attttgaaaa acagggttaa ttttagctga aaatcttcaa
      841 ttttgccaga aattggcttt ttttccgtcg ccacacggag aacttgccaa aaatgaagac
      901 ttttagccaa tttagccctg aaaattcgag ttttgggcga tttttggtct gagaagccgt
      961 tttcgcagta tattatattt tctcgttaaa tttcatttta aaataaacat tttgaacgta
     1021 taaccccaaa aattcgctgc gcaatggatg atttttgtcc aaagttttga taattttagc
     1081 taaaaacctc gaattttggc aatttttcgt gccgcatcgg ccgaaagttg actttttttg
     1141 gaattatcat aatttaatgc acattttggt agtttatctc acataatctg tctgcaggcg
     1201 ggaaaaattt gagataaact actaaaagat gcaaaacaag gatgataatt ccagaaaatc
     1261 aacttcggac cgtctcgaca cggaaaattg agcgaaattg gagttttaaa atgatttagc
     1321 cctgaaaatt cgagaaattt aggcggtttt gggtctgaaa aggcgttttc gcagtatttt
     1381 ctcgctaaat ttcatttatt atcacataat atcagtactt aggcgaatat cttgtagaaa
     1441 tgttggtttt gttcgataat tctctacttt tagagtcgat tcacgagaaa atgttgcacc
     1501 atcttacttt tttcccttgt aaaacataga ttcttctgta caatgaaaga gaacacaatt
     1561 tcttcgtgaa gcaactcaaa aagtagagaa aattcgaaca aaacccaaca tttctacaag
     1621 atattcgtct aaatagtgat ataatgtgtt aataagcata ttttaaatga aatttagcga
     1681 aaaaactgcg aaaatttgtc aaaaaagcga atttccatcc gatcagcaga tccctaaaag
     1741 ttacataaaa ttgaaagttt tgtggggaaa ttgttttaaa acacgcctat ggtaatacga
     1801 gaacataaaa ttctgagaat gcgtattaca caacatattc gacgcgcaaa atataactat
     1861 atctcgtagc gaaaactaca gtaatcctta aatgactact gtagcgattg tgtcgattta
     1921 cgggctcgat tctcggaaaa aaaaatcatt tttcaaattt tgacagcgat attcaatttt
     1981 tcttcgtttt ttcgtatttc tcagtcattt ttgtgcttat ttttaatatt ttattcatta
     2041 ataaattaat ttaattgaaa aacgtccaga cgtattttta catttcccgt ctatttccgt
     2101 aataggcggt taataacgta gaaatacgtc tgtatgagtt tactcatcaa aatgacacga
     2161 atattggacg tttttaaatc aaattaattt attaataggt aaaatattaa aaaataaaca
     2221 caaaaatgac aaaggaatag gaaaacacga agagaaattg agtatcgctg tcaaaattcg
     2281 aaaaacgtgg atttttccaa gaatcgagcc cgtaaatcga cacattcgct acagtagtca
     2341 tttaaggatt attgtagttt tcgctacgag atactttgcg cgccaaaaac gtggtgaaat
     2401 acgcattctc agaattttat gttcccgtaa taccacaatg accgaatatc ataataaaaa
     2461 aattcaaaaa caatttctag attttatatg attttctgaa aattgaaaaa tcatataaca
     2521 tctagacaat ttttttgaat ttttttatca tgatattcgg tcattgtgtc cccataggca
     2581 tgttttaaag caatttccac ctttaaataa aatcgagaaa aaatggcgtc aaaagacata
     2641 tgtaaaagct gtttctcttt gtatattcac acagaatata gcccgatttt ggaggtgaaa
     2701 aaccggtttt tttgtattct ttacgccaca taaagtgata tggaagagaa aatcgacata
     2761 tttatgtact tggagtacga gtacattgga gaaaaagggt gaactactgg aaaatctggg
     2821 aaatttcaga aaaaaattcc gaaaaatctt ttttgctgaa aaaaaaattt gttcgaaatt
     2881 tgggaaatac tggaaaattt tcatcaaaat taaagcgatt tttaacgttt ttgcttggga
     2941 gatactattt gtaattttaa tgtttggtga ggacaacaga aatgaccttc agcgtacgga
     3001 aaacgattaa aagacacatt tgaatcgaaa atagcgtgaa aaacatgaaa atatcggaaa
     3061 aatagcttta aaattggatt tgaaagcaaa aaatgtatca aaaatcgaga aaaatgcttg
     3121 aaaaagttga aaaactcaaa caaaaaatca aaaaaggttt ttttaaaaat ctgtgagtat
     3181 cgcaacgaaa gcacaacttg aggcatgcgc ctttaaacca accgtaacca aattcggagg
     3241 caaaaaaaga tttctcgctg ttttttcagt tttaaaatca cttttcacgt ttcttttcgc
     3301 agtaattcgt ttgcaaaaca tctgaaaaaa ttttatttta taagaaatta tataaaaatc
     3361 gctataaatt gagtttttgc ccccaaattc agcacggagc acttctcaat taaatataat
     3421 tttatttttt aacattgaaa gctttcataa aattgagctt tgtttgtaaa taatcagtga
     3481 aaaaacacag aaatcttcaa aaaataaaga aaaagttttt aaaaatctgt gagtcccgca
     3541 acgaaaataa aactttaggc atgcgccttt aaaccaaccg taaccaaatt cggcggcaaa
     3601 aatggatttc tcgccgtttt ttcagtttta caatcacttt ttaggtttct ttttgcagta
     3661 tttcgtttcc caaacaatta aaaatcaaat tttcttttcc agatggctca aacaaagccg
     3721 attgccgagc aaacaagttt gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat
     3781 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     3841 catatgcagt cactatgaat caactactta gatggtatta gtgacctgta gaattcgagc
     3901 tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc
     3961 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     4021 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     4081 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     4141 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     4201 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     4261 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     4321 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     4381 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     4441 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     4501 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     4561 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     4621 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     4681 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     4741 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     4801 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     4861 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     4921 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     4981 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5161 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5401 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     5461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
