LOCUS       sequence-85341-e        6028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6028)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Tensin1-N-18
FEATURES             Location/Qualifiers
     source          1..6028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5290..5299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5296..6006
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5709..5728
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgctagcgct accggacttc agatctcgag gccaccatga gtgtgagccg gaccatggag
       61 gacagctgtg agctggacct ggtgtacgtc acagagagga tcatcgctgt ctccttcccc
      121 agcacagcca atgaggagaa ctttcggagc aacctccgtg aggtggcgca gatgctcaag
      181 tccaaacatg gaggcaacta cctgctgttc aacctctctg agcggagacc tgacatcacg
      241 aagctccatg ccaaggtact ggaatttggc tggcccgacc tccacacccc agccctggag
      301 aagatatgca gcatctgtaa ggccatggac acatggctca atgcagaccc tcacaatgtc
      361 gttgttctac acaacaaggg aaaccgaggc aggataggag ttgtcatcgc ggcttacatg
      421 cactacagca acatttctgc cagtgcggac caggctctgg atcggtttgc aatgaagcgg
      481 ttctatgagg ataagattgt gcccattggc cagccatccc aaagaaggta cgtgcattac
      541 ttcagtggcc tgctctccgg ctccatcaaa atgaacaaca agcccttgtt tctgcaccac
      601 gtgatcatgc acggcatccc caactttgag tctaaaggag gatgtcggcc atttctccgc
      661 atctaccagg ccatgcaacc tgtgtacaca tctggcatct acaacatccc aggagacagc
      721 cagactagcg tctgcatcac catcgagcca ggactgctct tgaagggaga catcttgctg
      781 aagtgctacc acaagaagtt ccgaagccca gcccgagacg tcatcttccg tgtgcagttc
      841 cacacctgtg ccatccatga cctgggggtt gtctttggga aggaggacct tgatgatgct
      901 ttcaaagatg atcgatttcc agagtatggc aaagtggagt ttgtattttc ttatgggcca
      961 gagaaaattc aaggcatgga gcacctggag aacgggccga gcgtgtctgt ggactataac
     1021 acctccgacc ccctcatccg ctgggactcc tacgacaact tcagtgggca tcgagatgac
     1081 ggcatggagg aggtggtggg acacacgcag gggccactag atgggagcct gtatgctaag
     1141 gtgaagaaga aagactccct gcacggcagc accggggctg ttaatgccac acgtcctaca
     1201 ctgtcggcca cccccaacca cgtggaacac acgctttctg tgagcagcga ctcgggcaac
     1261 tccacagcct ccaccaagac cgacaagacc gacgagcctg tccccggggc ctccagtgcc
     1321 actgctgcct tgagtcccca ggagaagcgg gagctggacc gcctgctgag tggctttggc
     1381 ttagagcgag agaagcaagg cgccatgtac cacacccagc acctcaggtc ccgcccagca
     1441 gggggctcgg ctgtgccctc ctctggacgc cacgttgtcc cagcccaggt tcatgtcaat
     1501 ggtggggcgt tagcatctga gcgggagaca gacatcctgg acgatgaatt gccaaaccag
     1561 gatggtcaca gtgcgggcag catgggcaca ctctcttctc tggacggggt caccaacacc
     1621 agtgaggggg gctacccaga ggccctgtcc ccactgacca acggtctgga caagtcctac
     1681 cccatggagc ctatggtcaa tggaggaggc tacccctacg agtctgccag ccgggcgggg
     1741 cctgcccatg ctggccacac ggcccccatg cggccctcct actctgcaca ggagggttta
     1801 gctggctacc agagggaggg gccccaccca gcctggccac agccagtgac cacctcccac
     1861 tatgcccatg accccagcgg tatgttccgc tctcaatcct tttcggaagc tgaaccccag
     1921 ctgcccccag ctccggtccg agggggaagc agccgggagg ctgtgcaaag gggactgaac
     1981 tcgtggcagc agcagcagca gcagcagcag cagcctcgcc cacctccacg ccagcaggaa
     2041 agagcccact tggagagtct tgtagccagc aggcccagcc ctcagccatt ggcagagacc
     2101 cccatcccca gtctccctga gttcccgcga gcagcctccc agcaggagat tgaacagtcc
     2161 atcgaaacac tcaatatgct gatgctggac ctggagccag cctccgctgc tgccccacta
     2221 cacaagtccc agagtgtccc cggggcctgg ccaggggctt ctccactctc ctcccagccc
     2281 ctctctgggt cctcccgtca gtcccatcca ctgacccagt ccagatatgg ctatatcccc
     2341 agtgggcatt cgttgggaac ccctgagcca gccccacggg cctctctgga gtctgtccct
     2401 cctggcaggt cttactcacc ttatgactat cagccatgtt tggctgggcc taaccaggat
     2461 ttccattcaa agagcccagc ctcttcctcc ttgcctgcct tccttccgac cacccacagc
     2521 cctccagggc ctcagcaacc cccagcctct ctccctggcc tcactgctca gcctctgctc
     2581 tcaccaaagg aagcgacttc agacccctcc cggactccag aggaggagcc attgaattta
     2641 gaagggctgg tggcccacag ggtagcaggg gtacaggctc gggagaagca gcctgcagag
     2701 cccccagccc ctctgcggag gcgggcggcc agtgatggac agtatgagaa ccagtctcca
     2761 gaagccacat cccctcgtag ccctggggtt cgctcccctg tccagtgtgt ctccccggag
     2821 ctggctctta ccatcgctct caatcctgga gggcggccca aagagcccca tttgcacagc
     2881 tacaaggagg ccttcgagga gatggaggga acctccccga gcagcccacc acccagtggg
     2941 gtgcggtccc ccccgggtct ggccaagaca cccctgtctg ctctgggcct gaaacctcac
     3001 aacccagcgg acatcctgtt gcaccccaca ggagttacca gaagacggat tcagccagag
     3061 gaagatgagg ggaaggtggt ggtcaggctg tcagaagagc cccggagcta tgtggagtct
     3121 gtggcacgga cagcggtggc tggaccccga gctcaggact ctgagcccaa gagctttagt
     3181 gctccagcca cccaggccta tggccatgag atacccctga ggaacgggac cctgggtggc
     3241 tcctttgtct cccccagccc cctctccacc agcagcccca tcctcagtgc tgacagcact
     3301 tcagtgggga gtttcccgtc gggagagagc agtgaccagg gtccccggac gcccacccag
     3361 cctctgttgg agtctggctt ccgctcaggc agcctgggac agcccagccc gtctgcccag
     3421 agaaactacc agagctcttc tcctctcccg actgtgggca gtagctacag cagccccgac
     3481 tactcacttc agcatttcag ctcctctcca gaaagccagg ctcgcgctca gttcagtgtg
     3541 gctggcgtcc acacggtgcc tgggagccct caggcgcgcc acagaacagt gggcaccaac
     3601 actcccccta gtcctggctt cggctggcgg gccatcaatc ccagcatggc tgcccccagc
     3661 agtcccagtt tgagccatca ccagatgatg ggtccaccag gcactggctt ccatggtagc
     3721 actgtctcca gcccccagag cagtgcagcg accaccccgg ggagccccag cctgtgtcgg
     3781 cacccagcag gggtctacca ggtttctggc ctccacaaca aagtggccac caccccgggg
     3841 agtcccagcc tgggccggca ccctggggct caccaaggca acctggcctc cggtcttcat
     3901 agcaatgcaa tagccagccc tggaagcccc agcctgggcc gtcacctcgg agggtctgga
     3961 tctgtggttc ccggcagccc ctgcttggac cggcatgtgg cctatggtgg ctattctacc
     4021 ccggaggatc ggagacccac actgtcccgg cagagcagtg cctctggcta ccaggctcct
     4081 tccacgccct ccttccctgt ctcccctgcc tactaccctg gcctgagcag ccctgccacc
     4141 tccccgtcac cagactccgc agccttccgg caagggagcc caacaccagc cttgccagag
     4201 aagcgaagga tgtcagtggg agaccgggca ggcagcctcc ccaactatgc caccatcaat
     4261 gggaaggtgt cttcgcctgt cgccagcggc atgtccagtc ccagtggggg cagcaccgtc
     4321 tccttctccc acactctgcc cgacttctcc aagtactcca tgccagacaa cagcccggag
     4381 acgcgggcta aagtgaagtt tgtccaggac acttctaagt attggtacaa gcctgagatt
     4441 tccagggagc aggccatcgc gctcctcaag gaccaggagc cgggggcctt catcatccgc
     4501 gacagtcact ccttccgagg cgcgtacggg ctggccatga aggtgtcttc gccacctcca
     4561 accatcatgc agcagaataa aaaaggagac atgacccatg agctggtcag gcattttctg
     4621 atagagactg gccccagagg agtcaagctc aagggctgcc ccaatgagcc aaacttcgga
     4681 tcgctgtctg ccctggtcta ccagcactcc atcatcccat tggccctgcc ttgcaagctg
     4741 gtcattccaa accgagaccc cacagatgaa tcgaaagata gctccggccc tgccaactca
     4801 actgcagacc tgctgaaaca aggggcagcc tgcaatgtgc tcttcgtcaa ctctgtggac
     4861 atggagtcac tcactgggcc acaggccatc tctaaagcca catctgagac gttggctgca
     4921 gaccccacgc cagctgccac catcgttcac ttcaaagtct ctgcccaggg aatcactctg
     4981 actgacaacc agagaaagct ctttttcaga cgccactacc ctctcaacac tgtcaccttc
     5041 tgtgacctgg acccacagga aagaaagtgg atgaaaacag agggtggtgc ccctgctaag
     5101 ctcttcggct tcgtggcccg gaagcagggc agcaccacgg acaacgcctg ccacctcttt
     5161 gctgagcttg accccaacca gccggcctct gccatcgtca acttcgtctc caaggtcatg
     5221 ctgaatgccg gccaaaagag aggtagcgca tccgggggtg cgtcaggcgg cagcggatct
     5281 ggaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat catcaaggag
     5341 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     5401 ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa ggtgaccaag
     5461 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     5521 gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt ccccgagggc
     5581 ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt taaccaggac
     5641 tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     5701 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga ggagcggatg
     5761 taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct gaaggacggc
     5821 ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     5881 ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc
     5941 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6001 aagtaagcgg ccgcgactct agatcata
//
