LOCUS       sequence-85342-e        2119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2119)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-TGNP-N-10
FEATURES             Location/Qualifiers
     source          1..2119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1365..1374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1371..2081
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1784..1803
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc tatgccacca tgcggttcgt ggttgccttg gtcctcctga
       61 acgtcgcagc ggcgggagcc gtgccgctct tggccaccga aagcgtcaag caagaagaag
      121 ctggagtacg gccttctgca ggaaacgtct ccacccaccc cagcttgagc caacggcctg
      181 gaggctctac caagtcgcat ccggagccgc agactccaaa agacagccct agcaagtcga
      241 gtgcggaggc gcagacccca gaagacaccc ccaacaagtc gggtgcggag gcaaagaccc
      301 aaaaagacag ctccaacaag tcgggtgcgg aggcaaagac ccaaaaaggc agcactagca
      361 agtcgggttc ggaggcgcag accacaaaag acagcactag taagtcgcat ccggagctgc
      421 agactccaaa agacagcact ggcaaatcgg gtgcggaggc gcagacccca gaagacagcc
      481 ccaacaggtc gggtgcggag gcaaagaccc aaaaagacag ccctagcaag tcaggttcgg
      541 aggcgcagac cacaaaagat gtccctaata agtcgggtgc ggacggccag accccaaaag
      601 acggctccag caagtcgggt gcggaggatc agaccccaaa agacgtccct aacaagtcgg
      661 gtgcggagaa gcagactcca aaagacggct ctaacaagtc cggtgcagag gagcagggcc
      721 caatagacgg gcccagcaag tcgggtgcgg aggagcagac ctcaaaagac agccctaaca
      781 aggtggttcc agagcagcct tcctggaaag accattccaa gcccatctcc aacccttctg
      841 ataacaagga gctccccaag gctgacacaa accagcttgc tgacaaaggg aagctttctc
      901 ctcatgcttt caaaaccgaa tctggggagg aaactgacct catttctccc ccgcaggagg
      961 aagttaagtc ttcagagcct actgaggatg tggagcccaa agaggctgaa gatgatgata
     1021 caggacccga ggagggctca ccgcccaaag aagagaaaga aaagatgtcc ggttctgcct
     1081 ccagtgagaa ccgtgaagga acactttcgg attccacggg tagcgagaag gatgaccttt
     1141 atccgaacgg ttctggaaat ggcagcgcgg agagcagcca cttctttgca tatctggtga
     1201 ctgcagccat tcttgtggct gtcctctata tcgctcatca caacaagcgg aagatcattg
     1261 cttttgtcct ggaaggaaaa agatctaaag tcacccggcg gccaaaggcc agtgactacc
     1321 aacgtttgga ccagaagtcc agctccggag gggatccacc ggtcgccacc atggtgagca
     1381 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1441 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1501 aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1561 acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca
     1621 tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact
     1681 tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca
     1741 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1801 agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga
     1861 gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga
     1921 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
     1981 tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2041 gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg actctagatc
     2101 ataatcagcc ataccacat
//
