LOCUS       sequence-85343-e        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 568, 
            Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             487..1197
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     900..919
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttcagatccg ctagcgccac catggtgggt cggaacagcg ccatcgccgc cggtgtatgc
       61 ggggcccttt tcattgggta ctgcatctac ttcgaccgca aaagacgaag tgaccccaac
      121 ttcaagaaca ggcttcgaga acgaagaaag aaacagaagc ttgccaagga gagagctggg
      181 ctttccaagt tacctgacct taaagatgct gaagctgttc agaagttctt ccttgaagaa
      241 atacagcttg gtgaagagtt actagctcaa ggtgaatatg agaagggcgt agaccatctg
      301 acaaatgcaa ttgctgtgtg tggacagcca cagcagttac tgcaggtctt acagcaaact
      361 cttccaccac cagtgttcca gatgcttctg actaagctcc caacaattag tcagagaatt
      421 gtaagtgctc agagcttggc tgaagatgat gtggaaggcg gtagcgggga tccaccggtc
      481 gccaccatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc
      541 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      601 gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa gggtggcccc
      661 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggtctacatt
      721 aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg
      781 gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga ctcctccctg
      841 caggacggcg tgttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      901 cccgtaatgc agaagaagac catgggctgg gaggcctccg aggagcggat gtaccccgag
      961 gacggcgccc tgaagagcga gatcaagaag aggctgaagc tgaaggacgg cggccactac
     1021 gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     1081 atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat cgtggaacag
     1141 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg
     1201 gccgcgactc tagatcataa tcagccatac cac
//
