LOCUS       sequence-85344-e        2160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2160)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..739
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     445..464
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agaataacat
       61 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      121 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa
      181 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      241 catgtacggc tccaaggtct acattaagca cccagccgac atccccgact acttcaagct
      301 gtccttcccc gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat
      361 tcacgttaac caggactcct ccctgcagga cggcgtgttc atctacaagg tgaagctgcg
      421 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      481 ctccgaggag cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaagaggct
      541 gaagctgaag gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa
      601 gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa
      661 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      721 catggacgag ctgtacaagt ccggactcag atctcgagtg cgtgagtgca tctccatcca
      781 cgttggccag gctggtgtcc agattggcaa tgcctgctgg gagctctact gcctggaaca
      841 cggcatccag cccgatggcc agatgccaag tgacaagacc attgggggag gagatgactc
      901 cttcaacacc ttcttcagtg agacgggcgc tggcaagcac gtgccccggg ctgtgtttgt
      961 agacttggaa cccacagtca ttgatgaagt tcgcactggc acctaccgcc agctcttcca
     1021 ccctgagcag ctcatcacag gcaaggaaga tgctgccaat aactatgccc gagggcacta
     1081 caccattggc aaggagatca ttgaccttgt gttggaccga attcgcaagc tggctgacca
     1141 gtgcaccggt cttcagggct tcttggtttt ccacagcttt ggtgggggaa ctggttctgg
     1201 gttcacctcc ctgctcatgg aacgtctctc agttgattat ggcaagaagt ccaagctgga
     1261 gttctccatt tacccagcac cccaggtttc cacagctgta gttgagccct acaactccat
     1321 cctcaccacc cacaccaccc tggagcactc tgattgtgcc ttcatggtag acaatgaggc
     1381 catctatgac atctgtcgta gaaacctcga tatcgagcgc ccaacctaca ctaaccttaa
     1441 ccgccttatt agccagattg tgtcctccat cactgcttcc ctgagatttg atggagccct
     1501 gaatgttgac ctgacagaat tccagaccaa cctggtgccc tacccccgca tccacttccc
     1561 tctggccaca tatgcccctg tcatctctgc tgagaaagcc taccatgaac agctttctgt
     1621 agcagagatc accaatgctt gctttgagcc agccaaccag atggtgaaat gtgaccctcg
     1681 ccatggtaaa tacatggctt gctgcctgtt gtaccgtggt gacgtggttc ccaaagatgt
     1741 caatgctgcc attgccacca tcaaaaccaa gcgcagcatc cagtttgtgg attggtgccc
     1801 cactggcttc aaggttggca tcaactacca gcctcccact gtggtgcctg gtggagacct
     1861 ggccaaggta cagagagctg tgtgcatgct gagcaacacc acagccattg ctgaggcctg
     1921 ggctcgcctg gaccacaagt ttgacctgat gtatgccaag cgtgcctttg ttcactggta
     1981 cgtgggtgag gggatggagg aaggcgagtt ttcagaggcc cgtgaagata tggctgccct
     2041 tgagaaggat tatgaggagg ttggtgtgga ttctgttgaa ggagagggtg aggaagaagg
     2101 agaggaatac taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
