LOCUS       sequence-85345-e        2198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2198)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg cagcgcatcc
      781 ggcggaagcg gaagcgtgcg tgagtgcatc tccatccacg ttggccaggc tggtgtccag
      841 attggcaatg cctgctggga gctctactgc ctggaacacg gcatccagcc cgatggccag
      901 atgccaagtg acaagaccat tgggggagga gatgactcct tcaacacctt cttcagtgag
      961 acgggcgctg gcaagcacgt gccccgggct gtgtttgtag acttggaacc cacagtcatt
     1021 gatgaagttc gcactggcac ctaccgccag ctcttccacc ctgagcagct catcacaggc
     1081 aaggaagatg ctgccaataa ctatgcccga gggcactaca ccattggcaa ggagatcatt
     1141 gaccttgtgt tggaccgaat tcgcaagctg gctgaccagt gcaccggtct tcagggcttc
     1201 ttggttttcc acagctttgg tgggggaact ggttctgggt tcacctccct gctcatggaa
     1261 cgtctctcag ttgattatgg caagaagtcc aagctggagt tctccattta cccagcaccc
     1321 caggtttcca cagctgtagt tgagccctac aactccatcc tcaccaccca caccaccctg
     1381 gagcactctg attgtgcctt catggtagac aatgaggcca tctatgacat ctgtcgtaga
     1441 aacctcgata tcgagcgccc aacctacact aaccttaacc gccttattag ccagattgtg
     1501 tcctccatca ctgcttccct gagatttgat ggagccctga atgttgacct gacagaattc
     1561 cagaccaacc tggtgcccta cccccgcatc cacttccctc tggccacata tgcccctgtc
     1621 atctctgctg agaaagccta ccatgaacag ctttctgtag cagagatcac caatgcttgc
     1681 tttgagccag ccaaccagat ggtgaaatgt gaccctcgcc atggtaaata catggcttgc
     1741 tgcctgttgt accgtggtga cgtggttccc aaagatgtca atgctgccat tgccaccatc
     1801 aaaaccaagc gcagcatcca gtttgtggat tggtgcccca ctggcttcaa ggttggcatc
     1861 aactaccagc ctcccactgt ggtgcctggt ggagacctgg ccaaggtaca gagagctgtg
     1921 tgcatgctga gcaacaccac agccattgct gaggcctggg ctcgcctgga ccacaagttt
     1981 gacctgatgt atgccaagcg tgcctttgtt cactggtacg tgggtgaggg gatggaggaa
     2041 ggcgagtttt cagaggcccg tgaagatatg gctgcccttg agaaggatta tgaggaggtt
     2101 ggtgtggatt ctgttgaagg agagggtgag gaagaaggag aggaatacta aggatccacc
     2161 ggatctagat aactgatcat aatcagccat accacatt
//
